Donaghy Ludovic, Kim Bong-Kyu, Hong Hyun-Ki, Park Heung-Sik, Choi Kwang-Sik
School of Applied Marine Sciences (Post BK-21) and Marine and Environment Research Institute, Cheju National University, 66 Jejudaehakno, Jeju 690-756, Republic of Korea.
Fish Shellfish Immunol. 2009 Aug;27(2):296-301. doi: 10.1016/j.fsi.2009.05.010. Epub 2009 May 31.
The Suminoe oyster Crassostrea ariakensis has been attempted to be introduced in the Chesapeake Bay, USA, as an alternative to the eastern oyster Crassostrea virginica. Commercial production of Suminoe oysters is currently restricted due to the incomplete understanding of their biological, physiological and immunological nature. Accordingly, understanding immune system of C. ariakensis is crucial to prevent disease associated mortality and subsequent management of the Suminoe oyster. We investigated immunological activities and morphology of hemocytes of the Suminoe oyster using flow cytometry and light microscopy. Three types of hemocytes were identified in the hemolymph including hyalinocyte, granulocyte and blast-like cells. Hyalinocytes were the largest cells and the most abundant, while granulocytes were intermediate-size cell containing numerous granules in the cytoplasm. Blast-like cells were the smallest and least numerous. Flow cytometry revealed that the granulocytes are most active in the cell phagocytosis and spontaneous reactive oxygen species (ROS) production. The hyalinocytes also showed a certain level of the phagocytosis and oxidative activity but in a lesser extent than the granulocytes. In contrast, the blast-like cells did not show any phagocytosis or oxidative activity. The flow cytometry used in this study confirmed that as observed from other marine bivalves, the granulocytes are the main hemocytes involved in the cellular defence in the Suminoe oyster.
已尝试将日本牡蛎（Crassostrea ariakensis）引入美国切萨皮克湾，作为美国东牡蛎（Crassostrea virginica）的替代品。由于对其生物学、生理学和免疫学特性的了解尚不完整，目前日本牡蛎的商业生产受到限制。因此，了解日本牡蛎的免疫系统对于预防与疾病相关的死亡以及随后对日本牡蛎的管理至关重要。我们使用流式细胞术和光学显微镜研究了日本牡蛎血细胞的免疫活性和形态。在血淋巴中鉴定出三种类型的血细胞，包括透明细胞、颗粒细胞和类成血细胞。透明细胞是最大且数量最多的细胞，而颗粒细胞是中等大小的细胞，细胞质中含有许多颗粒。类成血细胞最小且数量最少。流式细胞术显示，颗粒细胞在细胞吞噬作用和自发活性氧（ROS）产生方面最为活跃。透明细胞也表现出一定程度的吞噬作用和氧化活性，但程度低于颗粒细胞。相比之下，类成血细胞未表现出任何吞噬作用或氧化活性。本研究中使用的流式细胞术证实，正如从其他海洋双壳类动物中观察到的那样，颗粒细胞是参与日本牡蛎细胞防御的主要血细胞。
