Kato Takayuki, Goodman Russell P, Erben Christoph M, Turberfield Andrew J, Namba Keiichi
Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan.
Nano Lett. 2009 Jul;9(7):2747-50. doi: 10.1021/nl901265n.
Many DNA nanostructures have been produced and a wide range of potential applications have been proposed. However, confirmation of accurate 3D construction is particularly challenging. Here, we demonstrate that cryoEM may be exploited to obtain structural information at sufficient resolution to visualize the DNA helix and reveal the absolute stereochemistry of a 7 nm self-assembled DNA tetrahedron. Structural analysis at such high resolution by cryoEM image analysis is unprecedented for any biological molecule of this size.
许多DNA纳米结构已经被制造出来，并且人们已经提出了广泛的潜在应用。然而，确认精确的三维结构尤其具有挑战性。在这里，我们证明了冷冻电镜可以被用来获得足够分辨率的结构信息，以可视化DNA螺旋并揭示一个7纳米自组装DNA四面体的绝对立体化学结构。通过冷冻电镜图像分析在如此高分辨率下进行结构分析，对于这种大小的任何生物分子来说都是前所未有的。
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