Liang Guo-Xi, Pan Hong-Cheng, Li Ye, Jiang Li-Ping, Zhang Jian-Rong, Zhu Jun-Jie
Key Lab. of Analytical Chemistry for Life Science (MOE), School of Chemistry and Chemical Engineering, Nanjing University, Hankou Road 22, Nanjing 210093, PR China.
Biosens Bioelectron. 2009 Aug 15;24(12):3693-7. doi: 10.1016/j.bios.2009.05.008. Epub 2009 May 14.
A novel sensing system based on the near infrared (NIR) fluorescence resonance energy transfer (FRET) between Mn:CdTe quantum dots (Qdots) and Au nanorods (AuNRs) was established for the detection of human IgG. The NIR-emitting Qdots linked with goat anti-human IgG (Mn:CdTe-Ab1) and AuNRs linked with rabbit anti-human IgG (AuNRs-Ab2) acted as fluorescence donors and acceptors, respectively. FRET occurred by human IgG with the specific antigen-antibody interaction. And human IgG was detected based on the modulation in FRET efficiency. The calibration graph was linear over the range of 0.05-2.5 microM of human IgG under optimal conditions. The proposed sensing system can decrease the interference of biomolecules in NIR region and increase FRET efficiency in optimizing the spectral overlap of AuNRs with Mn:CdTe Qdots. This method has great potential for multiplex assay with different donor-acceptor pairs.
建立了一种基于锰掺杂碲化镉量子点（Mn:CdTe Qdots）与金纳米棒（AuNRs）之间近红外（NIR）荧光共振能量转移（FRET）的新型传感系统，用于检测人免疫球蛋白G（IgG）。与山羊抗人IgG连接的近红外发射量子点（Mn:CdTe-Ab1）和与兔抗人IgG连接的金纳米棒（AuNRs-Ab2）分别作为荧光供体和受体。通过人IgG与特异性抗原-抗体相互作用发生FRET。并基于FRET效率的调制检测人IgG。在最佳条件下，人IgG浓度在0.05 - 2.5微摩尔范围内，校准曲线呈线性。所提出的传感系统可以减少近红外区域生物分子的干扰，并在优化金纳米棒与锰掺杂碲化镉量子点的光谱重叠时提高FRET效率。该方法在使用不同供体-受体对进行多重检测方面具有巨大潜力。
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