Zeng Qinghui, Li Qin, Ji Wenyu, Bin Xue, Song Jie
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Dong_Nanhu Road 3888, Changchun 130033, P. R. China.
Queensland Micro- and Nanotechnology Centre &Environmental Engineering, Griffith University, Brisbane, QLD 4111, Australia.
Sci Rep. 2016 May 20;6:26534. doi: 10.1038/srep26534.
With growing concerns about health issues worldwide, elegant sensors with high sensitivity and specificity for virus/antigens (Ag) detection are urgent to be developed. Homogeneous immunoassays (HIA) are an important technique with the advantages of small sample volumes requirement and pretreatment-free process. HIA are becoming more favorable for the medical diagnosis and disease surveillance than heterogeneous immunoassays. An important subset of HIA relies on the effect of fluorescence resonance energy transfer (FRET) via a donor-acceptor (D-A) platform, e.g., quantum dots (QDs) donor based FRET system. Being an excellent plasmonic material, silver triangular nanoplates (STNPs) have unique advantages in displaying surface plasmon resonance in the visible to near infrared spectral region, which make them a better acceptor for pairing with QDs in a FRET-based sensing system. However, the reported STNPs generally exhibited broad size distributions, which would greatly restrict their application as HIA acceptor for high detection sensitivity and specificity purpose. In this work, uniform STNPs and red-emitting QDs are firstly applied to construct FRET nanoplatform in the advanced HIA and further be exploited for analyzing virus Ag. The uniform STNPs/QDs nanoplatform based medical sensor provides a straightforward and highly sensitive method for Ag analysis in homogeneous form.
随着全球对健康问题的关注度不断提高，迫切需要开发出对病毒/抗原（Ag）检测具有高灵敏度和特异性的精密传感器。均相免疫分析（HIA）是一项重要技术，具有所需样品量少且无需预处理的优点。与异相免疫分析相比，HIA在医学诊断和疾病监测中越来越受到青睐。HIA的一个重要子集依赖于通过供体-受体（D-A）平台的荧光共振能量转移（FRET）效应，例如基于量子点（QDs）供体的FRET系统。作为一种优异的等离子体材料，银三角纳米片（STNPs）在可见光到近红外光谱区域显示表面等离子体共振方面具有独特优势，这使其成为基于FRET的传感系统中与量子点配对的更好受体。然而，报道的STNPs通常表现出较宽的尺寸分布，这将极大地限制其作为HIA受体用于高检测灵敏度和特异性目的的应用。在这项工作中，首先将均匀的STNPs和发红光的量子点应用于构建先进HIA中的FRET纳米平台，并进一步用于分析病毒Ag。基于均匀STNPs/QDs纳米平台的医学传感器为均相形式的Ag分析提供了一种直接且高度灵敏的方法。
