Yarom N, Shani T, Amariglio N, Taicher S, Kaplan I, Vered M, Rechavi G, Trakhtenbrot L, Hirshberg A
Department of Oral and Maxillofacial Surgery, The Chaim Sheba Medical Center, Tel Hashomer, Israel.
J Dent Res. 2009 May;88(5):427-32. doi: 10.1177/0022034509337089.
The malignant potential of oral lichen planus (OLP) has been a matter of serious controversy. We aimed to detect chromosomal numerical aberrations in cells of brush samples collected from affected mucosa. The samples were simultaneously analyzed for morphology and fluorescent in situ hybridization (FISH) with chromosomes 2 and 8 centromeric probes. We analyzed 57 persons with OLP and 33 control individuals. A cut-off value of aneuploid cells was determined as 1.1%. Aneuploid cells were found in 16 persons with OLP (28.1%); in 10 individuals (17.5%), over 5% of the cells were aneuploid. Aneuploid cells were also detected in normal-looking mucosa of seven persons with OLP. One person with OLP developed squamous cell carcinoma; 10% of the cells examined were aneuploid. OLP carries an increased risk for chromosomal instability. Identifying aneuploid cells in a brush sample and the combined morphological and FISH analysis can increase the specificity in predicting the malignant potential of OLP.
口腔扁平苔藓（OLP）的恶性潜能一直是一个备受争议的问题。我们旨在检测从受影响黏膜采集的刷检样本细胞中的染色体数目畸变。对样本同时进行形态学分析以及使用2号和8号染色体着丝粒探针进行荧光原位杂交（FISH）分析。我们分析了57例OLP患者和33名对照个体。非整倍体细胞的临界值确定为1.1%。在16例OLP患者（28.1%）中发现了非整倍体细胞；在10名个体（17.5%）中，超过5%的细胞为非整倍体。在7例OLP患者看似正常的黏膜中也检测到了非整倍体细胞。1例OLP患者发展为鳞状细胞癌；所检查的细胞中有10%为非整倍体。OLP存在染色体不稳定风险增加的情况。在刷检样本中识别非整倍体细胞以及联合形态学和FISH分析可提高预测OLP恶性潜能的特异性。
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