Perry Stuart T, Prestwood Tyler R, Lada Steven M, Benedict Chris A, Shresta Sujan
La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA.
J Virol. 2009 Aug;83(16):8276-81. doi: 10.1128/JVI.00365-09. Epub 2009 Jun 3.
The role of Cardif-dependent signaling in controlling dengue virus (DENV) infection and regulating type I interferon (IFN) production in vivo was examined in Cardif-deficient mice. DENV RNA levels were significantly elevated in both the serum and lymphoid tissues of Cardif(-/-) mice at early times compared to those in wild-type animals. Type I IFN production was delayed in these locales of Cardif(-/-) mice until 18 h postinfection, indicating that Cardif regulates the initial type I IFN response in lymphoid tissues. In contrast, DENV viral loads in nonlymphoid tissues were similar between Cardif(-/-) and wild-type mice. These results reveal that RNA helicase-mediated sensing acts as a first line of innate defense against DENV infection in vivo and functions in a tissue-dependent manner.
在Cardif基因缺陷小鼠中研究了依赖Cardif的信号传导在体内控制登革病毒（DENV）感染和调节I型干扰素（IFN）产生中的作用。与野生型动物相比，在早期，Cardif（-/-）小鼠的血清和淋巴组织中的DENV RNA水平均显著升高。Cardif（-/-）小鼠这些部位的I型干扰素产生延迟至感染后18小时，表明Cardif调节淋巴组织中的初始I型干扰素反应。相比之下，Cardif（-/-）小鼠和野生型小鼠非淋巴组织中的DENV病毒载量相似。这些结果表明，RNA解旋酶介导的传感作为体内针对DENV感染的先天性防御的第一道防线，并以组织依赖的方式发挥作用。
