Jeon Young Min, Kim Bong Gyu, Ahn Joong-Hoon
Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea.
J Microbiol Biotechnol. 2009 May;19(5):491-4. doi: 10.4014/jmb.0807.402.
Within the secondary metabolite class of flavonoids, which consist of more than 10,000 known structures, flavones define one of the largest subgroups. The diverse function of flavones in plants as well as their various roles in the interaction with other organisms offers many potential applications including in human nutrition and pharmacology. We used two genes, flavone synthase (PFNS-1) that converts naringenin into apigenin and a flavone 7-O-methyltransferase gene (POMT-7) that converts apigenin into 7-O-methyl apigenin, to synthesize 7-O-methyl apigenenin from naringenin. The PFNS-1 gene was subcloned into the E. coli expression vector pGEX and POMT-7 was subcloned into the pRSF vector. Since both constructs contain different replication origins and selection markers, they were cotransformed into E. coli. Using E. coli transformants harboring both PFNS-1 and POMT-7, naringenin could be converted into 7-O-methyl apigenin, genkwanin.
在黄酮类次生代谢产物类别中，已知结构超过10000种，黄酮是最大的亚组之一。黄酮在植物中的多种功能以及它们在与其他生物体相互作用中的各种作用提供了许多潜在应用，包括在人类营养和药理学方面。我们使用了两个基因，将柚皮素转化为芹菜素的黄酮合酶（PFNS-1）和将芹菜素转化为7-O-甲基芹菜素的黄酮7-O-甲基转移酶基因（POMT-7），从柚皮素合成7-O-甲基芹菜素。将PFNS-1基因亚克隆到大肠杆菌表达载体pGEX中，将POMT-7亚克隆到pRSF载体中。由于这两个构建体包含不同的复制起点和选择标记，它们被共转化到大肠杆菌中。利用同时携带PFNS-1和POMT-7的大肠杆菌转化体，柚皮素可以转化为7-O-甲基芹菜素，即芫花素。
