Bernadina W E, van Leeuwen M A, Hendrikx W M, Ruitenberg E J
Department of Immunology, Faculty of Veterinary Medicine, Utrecht, Netherlands.
Vet Immunol Immunopathol. 1991 Aug;29(1-2):127-38. doi: 10.1016/0165-2427(91)90058-k.
Neutrophil (PMN) counts, immune complex (IC) uptake by PMN, and serum opsonising activity for promoting yeast uptake were used to evaluate infection clearing capacity in 16 lambs prior to colostrum feeding (two lambs fed bovine colostrum, 14 suckled lambs) and at 2 days of age. At 2 days of age lambs had more circulating PMN than they had prior to colostrum uptake (P less than 0.01). Colostrum feeding caused a significant increase in the percent of lamb PMN phagocytosing IC, although at Day 2 the percent phagocytosis was significantly lower (32.2%) than for adult controls (90%). Yeast opsonophagocytosis was greater when 24-36 h post-feeding serum was the source of opsonin than when pre-feeding serum was used (P less than 0.001). When adult serum was the opsonin, yeast opsonophagocytosis was approximately twice the phagocytosis mediated by 24-36 h post-feeding serum. The peripheral neutrocytosis and the enhancement of opsonophagocytosis generated by absorption of either ovine or bovine colostrum did not differ. The results of this study suggest that the parameters evaluated may be used for indicating the presence (or absence) of passively acquired protective immunity.
在初乳喂养前（2只喂牛初乳的羔羊，14只吮乳羔羊）及2日龄时，通过检测中性粒细胞（PMN）计数、PMN对免疫复合物（IC）的摄取以及促进酵母摄取的血清调理活性，来评估16只羔羊的感染清除能力。2日龄时羔羊循环中的PMN比摄取初乳前更多（P<0.01）。初乳喂养使羔羊PMN吞噬IC的百分比显著增加，尽管在第2天吞噬百分比（32.2%）显著低于成年对照组（90%）。当以喂食后24 - 36小时的血清作为调理素来源时，酵母调理吞噬作用比使用喂食前血清时更强（P<0.001）。当以成年血清作为调理素时，酵母调理吞噬作用约为喂食后24 - 36小时血清介导的吞噬作用的两倍。吸收绵羊或牛初乳所产生的外周中性粒细胞增多和调理吞噬作用增强并无差异。本研究结果表明，所评估的参数可用于指示被动获得性保护性免疫的存在（或不存在）。
