Kostro K, Wiktorowicz K
Clinic of Infectious Diseases of Animals, Agricultural Academy, Lublin, Poland.
Vet Immunol Immunopathol. 1991 Aug;29(1-2):183-8. doi: 10.1016/0165-2427(91)90063-i.
The proliferative response of fox peripheral blood lymphocytes to nonspecific mitogens: leucoagglutinin (LA), concanavalin A (Con A) and pokeweed mitogen (PWM) was studied. Microcultures were kept at 39 degrees C in a humidified atmosphere containing 5% CO2. The highest 3H-thymidine incorporation was observed, when Con A was used, while LA and PWM showed weaker but significant stimulatory action. Optimal doses of mitogens were: 5 micrograms/ml for Con A, 5 micrograms/ml for LA and a dilution of 1:100 for PWM. The maximal stimulation index for Con A was about 240 and up to 100 for LA or PWM. The maximal lymphocyte proliferation was observed when culture media were supplemented with 10% serum. When proliferation kinetics were studied, the peak response was observed on Day 2.
白细胞凝集素（LA）、刀豆球蛋白A（Con A）和商陆有丝分裂原（PWM）的增殖反应。微培养物在含有5%二氧化碳的潮湿气氛中于39摄氏度保存。当使用Con A时，观察到最高的3H-胸腺嘧啶核苷掺入，而LA和PWM显示出较弱但显著的刺激作用。有丝分裂原的最佳剂量为：Con A 5微克/毫升，LA 5微克/毫升，PWM 1:100稀释。Con A的最大刺激指数约为240，LA或PWM高达100。当培养基补充10%血清时，观察到最大淋巴细胞增殖。当研究增殖动力学时，在第2天观察到峰值反应。
