Reassessment of two Boyden chamber methods for measuring canine neutrophil migration: the leading front and the lower surface count assays.

Laboratory investigation of neutrophil locomotion has attracted a great deal of attention owing to its potential usefulness to veterinary clinical medicine. Two of the most important principles for measurement of chemotaxis are the leading front and lower surface count techniques. The latter assay has become increasingly popular following the introduction of multi-well chambers utilizing polycarbonate filters. In the present study, this method was compared quantitatively and qualitatively to the leading front assay. Further, the potential usefulness of a simple shape-change assay as a rapid measure of chemotactic activation of neutrophils was assessed and compared with the migration assays. It was concluded that the two migration assays are superior to the shape-change assay, even though both suffer from certain methodological drawbacks. This may be relevant to the elucidation of clinical cases of neutrophil dysfunction.