通过等电聚焦法对猪血浆和横纹肌中肌酸激酶-MM同工型的鉴定。

Identification by isoelectric focusing of creatine kinase-MM isoforms in the plasma and striated muscle of pigs.

作者信息

Doizé F, Deroth L

机构信息

Laboratory of Comparative Biomedicine, University of Montreal, Faculty of Veterinary Medicine, St-Hyacinthe, Quebec, Canada.

出版信息

Vet Res Commun. 1991;15(4):279-84. doi: 10.1007/BF00430032.

DOI:10.1007/BF00430032

PMID:1949600

Abstract

Total creatine kinase (CK) and CK-MM isoforms were determined in plasma and longissimus dorsi muscle extracts from normal pigs. Based on their total CK activity, the pigs were divided into two groups. Pigs of group 1 (n = 16) had a mean plasma total CK of 298 +/- 16 U/L and the distribution of the CK-MM isoforms was 65.7 +/- 2.5% CK-MM3, 18.9 +/- 1.6% CK-MM2 and 15.3 +/- 1.5% CK-MM. In group 2 (n = 18; 826 +/- 75 U/L total CK) four isoforms were observed: 3.1 +/- 0.9% CK-MM, 67.9 +/- 3.0% CK-MM3, 21.5 +/- 2.3% CK-MM2 and 7.5 +/- 1.3% CK-MM. The differences between the two groups of pigs were significant (p less than 0.001) for CK-MM1 and the presence of CK-MM. Four CK-MM isoforms were also detected in longissimus dorsi muscle homogenates: 45.6 +/- 8.1% CK-MM, 32.6 +/- 11.7% CK-MM3, 16.6 +/- 2.3% CK-MM2 and 5.1 +/- 2.8% CK-MM1. The release of CK-MM isoforms from muscle into plasma seems to be unrelated to the concentration of these isoforms in striated muscle.

摘要

测定了正常猪血浆和背最长肌提取物中的总肌酸激酶（CK）及CK-MM同工酶。根据总CK活性，将猪分为两组。第1组猪（n = 16）的血浆总CK平均为298±16 U/L，CK-MM同工酶的分布为：CK-MM3占65.7±2.5%，CK-MM2占18.9±1.6%，CK-MM1占15.3±1.5%。第2组猪（n = 18；总CK为826±75 U/L）观察到四种同工酶：CK-MM1占3.1±0.9%，CK-MM3占67.9±3.0%，CK-MM2占21.5±2.3%，CK-MM1占7.5±1.3%。两组猪之间CK-MM1及CK-MM1的存在差异显著（p＜0.001）。在背最长肌匀浆中也检测到四种CK-MM同工酶：CK-MM1占45.6±8.1%，CK-MM3占32.6±11.7%，CK-MM2占16.6±2.3%，CK-MM1占5.1±2.8%。CK-MM同工酶从肌肉释放到血浆中似乎与这些同工酶在横纹肌中的浓度无关。