Chen Chuanfang, Cui Yuanbo, Yue Jiachang, Huo Xiaolin, Song Tao
Institute of Electrical Engineering, Chinese Academy of Sciences, Beijing, China.
Bioelectromagnetics. 2009 Dec;30(8):663-8. doi: 10.1002/bem.20509.
The effects of extremely low frequency (ELF) magnetic fields on membrane F(0)F(1)-ATPase activity have been studied. When the F(0)F(1)-ATPase was exposed to 60 Hz magnetic fields of different magnetic intensities, 0.3 and 0.5 mT magnetic fields enhanced the hydrolysis activity, whereas 0.1 mT exposure caused no significant changes. Even if the F(0)F(1)-ATPase was inhibited by N,N-dicyclohexylcarbodiimide, its hydrolysis activity was enhanced by a 0.5 mT 60 Hz magnetic field. Moreover, when the chromatophores which were labeled with F-DHPE were exposed to a 0.5 mT, 60 Hz magnetic field, it was found that the pH of the outer membrane of the chromatophore was unchanged, which suggested that the magnetic fields used in this work did not affect the activity of F0. Taken together, our results show that the effects of magnetic fields on the hydrolysis activity of the membrane F(0)F(1)-ATPases were dependent on magnetic intensity and the threshold intensity is between 0.1 and 0.3 mT, and suggested that the F1 part of F(0)F(1)-ATPase may be an end-point affected by magnetic fields.
极低频（ELF）磁场对膜F(0)F(1)-ATP酶活性的影响已得到研究。当F(0)F(1)-ATP酶暴露于不同磁场强度的60Hz磁场中时，0.3和0.5mT的磁场增强了水解活性，而暴露于0.1mT时未引起显著变化。即使F(0)F(1)-ATP酶被N,N-二环己基碳二亚胺抑制，其水解活性仍被0.5mT的60Hz磁场增强。此外，当用F-DHPE标记的载色体暴露于0.5mT、60Hz的磁场中时，发现载色体外膜的pH值未发生变化，这表明本研究中使用的磁场不影响F0的活性。综上所述，我们的结果表明，磁场对膜F(0)F(1)-ATP酶水解活性的影响取决于磁场强度，阈值强度在0.1至0.3mT之间，并表明F(0)F(1)-ATP酶的F1部分可能是受磁场影响的终点。
