F(1)F(0) ATP合酶的a亚基需要YidC和SecYEG来进行膜插入。
Subunit a of the F(1)F(0) ATP synthase requires YidC and SecYEG for membrane insertion.
作者信息
Kol Stefan, Majczak Wiktor, Heerlien René, van der Berg Jan Pieter, Nouwen Nico, Driessen Arnold J M
机构信息
Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands.
出版信息
J Mol Biol. 2009 Jul 31;390(5):893-901. doi: 10.1016/j.jmb.2009.05.074. Epub 2009 Jun 1.
The insertion of inner membrane proteins in Escherichia coli occurs almost exclusively via the SecYEG pathway, while some membrane proteins require the membrane protein insertase YidC. In vitro analysis demonstrates that subunit a of the F(1)F(0) ATP synthase (F(0)a) is strictly dependent on Ffh, SecYEG and YidC for its membrane insertion but independent of the proton motive force. The insertion of the first transmembrane segment of F(0)a also depends on Ffh and SecYEG but not on YidC, whereas the insertion is strongly dependent on the proton motive force, unlike the full-length F(0)a protein. These data demonstrate an extensive role of YidC in the assembly of the F(0) sector of the F(1)F(0) ATP synthase.
大肠杆菌内膜蛋白的插入几乎完全通过SecYEG途径发生，而一些膜蛋白需要膜蛋白插入酶YidC。体外分析表明，F(1)F(0)ATP合酶的a亚基（F(0)a）的膜插入严格依赖于Ffh、SecYEG和YidC，但与质子动力势无关。F(0)a第一个跨膜片段的插入也依赖于Ffh和SecYEG，但不依赖于YidC，而与全长F(0)a蛋白不同，该插入强烈依赖于质子动力势。这些数据证明了YidC在F(1)F(0)ATP合酶F(0)扇区组装中的广泛作用。
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