du Plessis David J F, Nouwen Nico, Driessen Arnold J M
Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Materials Science Center Plus, University of Groningen, 9751 NN Haren, The Netherlands.
J Biol Chem. 2006 May 5;281(18):12248-52. doi: 10.1074/jbc.M600048200. Epub 2006 Mar 2.
The Escherichia coli YidC protein belongs to the Oxa1 family of membrane proteins that facilitate the insertion of membrane proteins. Depletion of YidC in E. coli leads to a specific defect in the functional assembly of major energy transducing complexes such as the F1F0 ATPase and cytochrome bo3 oxidase. Here we report on the in vitro reconstitution of the membrane insertion of the CyoA subunit of cytochrome bo3 oxidase. Efficient insertion of in vitro synthesized pre-CyoA into proteoliposomes requires YidC, SecYEG, and SecA and occurs independently of the proton motive force. These data demonstrate that pre-CyoA is a substrate of a novel pathway that involves both SecYEG and YidC.
大肠杆菌YidC蛋白属于促进膜蛋白插入的Oxa1膜蛋白家族。大肠杆菌中YidC的缺失会导致主要能量转换复合物（如F1F0 ATP酶和细胞色素bo3氧化酶）功能组装的特定缺陷。在此，我们报道了细胞色素bo3氧化酶CyoA亚基膜插入的体外重建。体外合成的前CyoA高效插入蛋白脂质体需要YidC、SecYEG和SecA，且其发生与质子动力无关。这些数据表明，前CyoA是一种涉及SecYEG和YidC的新途径的底物。
