使用阶梯式聚合酶链反应引物的多重荧光单链构象多态性分析
Multiplex, fluorescent single-strand conformation polymorphism using stepped polymerase chain reaction primers.
作者信息
Scholl R, Walker A, Ballard L W
机构信息
Genomics Core Facility, University of Utah, Salt Lake City, UT 84132, USA.
出版信息
J Biomol Tech. 2001 Mar;12(1):1-3.
Abstract
A method for high-throughput screening for a well-characterized mutation at the APC locus was developed using stepped PCR primers, pooled product, and fluorescent single-strand conformation polymorphism (SSCP). Using a panel of two known normals, three known heterozygous mutants, and one known homozygous mutant, eight stepped PCR products were developed that showed the same mobility shift on a PE Biosystems 373A. Using this method, up to eight different samples can be pooled for same lane analysis.
摘要
利用阶梯式PCR引物、混合产物和荧光单链构象多态性（SSCP），开发了一种用于高通量筛选APC基因座特征明确突变的方法。使用一组两个已知的正常样本、三个已知的杂合突变体和一个已知的纯合突变体，开发了八个阶梯式PCR产物，它们在PE Biosystems 373A上显示出相同的迁移率变化。使用这种方法，最多可以将八个不同的样本混合进行同泳道分析。
相似文献
1
Multiplex, fluorescent single-strand conformation polymorphism using stepped polymerase chain reaction primers.使用阶梯式聚合酶链反应引物的多重荧光单链构象多态性分析
J Biomol Tech. 2001 Mar;12(1):1-3.
2
Multiple Fluorescence-Based PCR-SSCP Analysis with Primer-, Post-, and Internal-Labeling.基于多重荧光的引物标记、后标记和内标记PCR-SSCP分析
Methods Mol Med. 1998;16:51-60. doi: 10.1385/0-89603-499-2:51.
3
Fluorescence-based mutation detection. Single-strand conformation polymorphism analysis (F-SSCP).基于荧光的突变检测。单链构象多态性分析（F-SSCP）。
Mol Biotechnol. 1996 Feb;5(1):17-31. doi: 10.1007/BF02762409.
4
Determination of the genome composition of influenza virus reassortants using multiplex reverse transcription-polymerase chain reaction followed by fluorescent single-strand conformation polymorphism analysis.采用多重逆转录-聚合酶链反应及荧光单链构象多态性分析来确定流感病毒重配体的基因组组成。
Anal Biochem. 1997 Oct 1;252(1):24-32. doi: 10.1006/abio.1997.2269.
5
An efficient strategy for detection of known and new mutations of the CYP2D6 gene using single strand conformation polymorphism analysis.一种使用单链构象多态性分析检测CYP2D6基因已知和新突变的有效策略。
Pharmacogenetics. 1995 Dec;5(6):373-84. doi: 10.1097/00008571-199512000-00006.
6
High-throughput single nucleotide polymorphism typing by fluorescent single-strand conformation polymorphism analysis with capillary electrophoresis.通过毛细管电泳荧光单链构象多态性分析进行高通量单核苷酸多态性分型
Electrophoresis. 2004 Mar;25(6):833-8. doi: 10.1002/elps.200305721.
7
A rapid fluorescence based multiplex polymerase chain reaction--single-strand conformation polymorphism method for p53 mutation detection.一种基于快速荧光的多重聚合酶链反应——单链构象多态性方法用于p53突变检测。
Electrophoresis. 2000 Jul;21(12):2335-42. doi: 10.1002/1522-2683(20000701)21:12<2335::AID-ELPS2335>3.0.CO;2-2.
8
Prenatal diagnosis of steroid 21-hydroxylase deficiency by analysis of polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) profiles.通过聚合酶链反应-单链构象多态性（PCR-SSCP）分析进行类固醇21-羟化酶缺乏症的产前诊断。
Prenat Diagn. 1997 May;17(5):435-42. doi: 10.1002/(sici)1097-0223(199705)17:5<435::aid-pd100>3.0.co;2-z.
9
Molecular identification of bacteria by fluorescence-based PCR-single-strand conformation polymorphism analysis of the 16S rRNA gene.基于荧光的16S rRNA基因PCR-单链构象多态性分析对细菌进行分子鉴定。
J Clin Microbiol. 1995 Oct;33(10):2601-6. doi: 10.1128/jcm.33.10.2601-2606.1995.
10
Analytical evaluation of primer engineered multiplex polymerase chain reaction-restriction fragment length polymorphism for detection of factor V Leiden and prothrombin G20210A.用于检测凝血因子V莱顿突变和凝血酶原G20210A的引物工程化多重聚合酶链反应-限制性片段长度多态性分析评估
J Mol Diagn. 2000 Aug;2(3):153-7. doi: 10.1016/S1525-1578(10)60631-9.
引用本文的文献
1
SSCP-SNP in pearl millet--a new marker system for comparative genetics.珍珠粟中的单链构象多态性-单核苷酸多态性——一种用于比较遗传学的新标记系统
Theor Appl Genet. 2005 May;110(8):1467-72. doi: 10.1007/s00122-005-1981-0. Epub 2005 Apr 5.
本文引用的文献
1
Multiplex mutation screening of the BRCA1 gene in 1000 Japanese breast cancers.对1000例日本乳腺癌患者的BRCA1基因进行多重突变筛查。
Jpn J Cancer Res. 1998 Jan;89(1):12-6. doi: 10.1111/j.1349-7006.1998.tb00472.x.
2
Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping.Taq DNA聚合酶对非模板核苷酸添加的调控：有助于基因分型的引物修饰
Biotechniques. 1996 Jun;20(6):1004-6, 1008-10. doi: 10.2144/96206st01.
3
Non-radioactive multiplex-SSCP analysis: detection of a new type II procollagen gene (COL2A1) mutation.
Hum Genet. 1995 Apr;95(4):437-9. doi: 10.1007/BF00208971.
4
Multiplex PCR-SSCP for simultaneous screening for mutations in several exons of p53.
Biotechniques. 1995 May;18(5):742-4.
5
Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction.利用聚合酶链反应快速灵敏地检测点突变和DNA多态性。
Genomics. 1989 Nov;5(4):874-9. doi: 10.1016/0888-7543(89)90129-8.
6
F-SSCP: fluorescence-based polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis.F-SSCP：基于荧光的聚合酶链反应-单链构象多态性（PCR-SSCP）分析
PCR Methods Appl. 1992 Aug;2(1):10-3. doi: 10.1101/gr.2.1.10.
Zotero 插件