Wu Bing, Cheng Shupei, Li Yiqiang, Kong Jie, Zhao Dayong, Zhang Yan, Zhang Xuxiang
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, 210093 Nanjing, China.
Ecotoxicology. 2009 Aug;18(6):715-21. doi: 10.1007/s10646-009-0339-9. Epub 2009 Jun 5.
In order to assess the potential health effects of source water from the Yangtze River at Nanjing section, China, hepatic transcriptional profiles of male mice (Mus musculus) exposed to source water for 90 days were measured with Affymetrix Mouse Genome 430A 2.0 Array. A total of 585 gene expressions were significantly altered (1.5-fold, P < or = 0.05), including 298 up-regulated genes and 287 down-regulated genes. Among the identified genes, potentially important genes that may be implicated in the liver cancer were found, including VCAM 1, Dusp1, Cyp7a1, Egfr and Fhit. The source water exposure also resulted in significant aberration of gene expressions and biological pathways linking to xenobiotic metabolism, signal transduction, cell growth and death, immune/inflammation response and oxidative stress response. The results provide excellent insights into early toxic effects of the Yangtze River source water on human and environmental health.
为评估中国长江南京段水源水对健康的潜在影响，利用Affymetrix Mouse Genome 430A 2.0芯片检测了雄性小鼠（小家鼠）暴露于该水源水90天后的肝脏转录谱。共有585个基因表达发生显著变化（变化1.5倍，P≤0.05），其中298个基因上调，287个基因下调。在所鉴定的基因中，发现了可能与肝癌相关的潜在重要基因，包括血管细胞黏附分子1（VCAM 1）、双特异性磷酸酶1（Dusp1）、细胞色素P450 7A1（Cyp7a1）、表皮生长因子受体（Egfr）和脆性组氨酸三联体基因（Fhit）。暴露于该水源水还导致与异生物质代谢、信号转导、细胞生长与死亡、免疫/炎症反应以及氧化应激反应相关的基因表达和生物途径出现显著异常。这些结果为长江水源水对人类和环境健康的早期毒性效应提供了重要见解。
