State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, China.
Hum Exp Toxicol. 2010 Feb;29(2):113-20. doi: 10.1177/0960327109357776. Epub 2009 Dec 22.
In order to evaluate the environmental health risk of drinking water from Yangtze River source, randomly amplified polymorphic DNA (RAPD) markers were used to detect the effects of the source water on genomic polymorphisms of hepatic cell of male mice (Mus musculus, ICR). After the mice were fed with source water for 90 days, RAPD-polymerase chain reactions (PCRs) were performed on hepatic genomic DNA using 20 arbitrary primers. Totally, 189 loci were generated, including 151 polymorphic loci. On average, one PCR primer produced 5.3, 4.9 and 4.8 bands for each mouse in the control, the groups fed with source water and BaP solution, respectively. Compared with the control, feeding mice with Yangtze River source water caused 33 new loci to appear and 19 to disappear. Statistical analysis of RAPD printfingers revealed that Yangtze River source water exerted a significant influence on the hepatic genomic polymorphisms of male mice. This study suggests that RAPD is a reliable and sensitive method for the environmental health risk of Yangtze River source water.
为了评估长江源水的环境卫生健康风险，本研究采用随机扩增多态性 DNA（RAPD）标记技术，检测了水源水对雄性昆明种小白鼠（Mus musculus，ICR）肝细胞基因组多态性的影响。受试雄性小白鼠连续 90 天饮用源水后，应用 20 个随机引物对其肝基因组 DNA 进行 RAPD-PCR 扩增，共得到 189 个位点，其中多态性位点 151 个。平均每个引物在对照组、源水组和 BaP 溶液组的每只小白鼠中共产生 5.3、4.9 和 4.8 条带。与对照组相比，饮用长江源水的小白鼠有 33 个新的位点出现，19 个位点消失。RAPD 指纹图谱的统计分析表明，长江源水对雄性小白鼠的肝基因组多态性有显著影响。该研究提示 RAPD 是一种检测长江源水环境卫生健康风险的可靠而敏感的方法。
