3-（10'-吩噻嗪基）丙烷-1-磺酸盐是大豆过氧化物酶诱导化学发光的强效增强剂。
3-(10'-Phenothiazinyl)propane-1-sulfonate is a potent enhancer of soybean peroxidase-induced chemiluminescence.
作者信息
Vdovenko Marina M, Ciana Leopoldo Della, Sakharov Ivan Yu
机构信息
Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.
出版信息
Anal Biochem. 2009 Sep 1;392(1):54-8. doi: 10.1016/j.ab.2009.05.031. Epub 2009 Jun 3.
3-(10'-Phenothiazinyl)propane-1-sulfonate (SPTZ) was shown to be a potent enhancer of soybean peroxidase (SbP)-induced chemiluminescence. To the best of our knowledge, SPTZ is the first enhancer of SbP to be discovered. Optimal conditions for SbP-catalyzed oxidation of luminol in the presence of SPTZ were determined. The SbP-SPTZ system showed better sensitivity and a lower detection limit (LDL) with respect to the horseradish peroxidase-4-iodophenol system traditionally used in chemiluminescent enzyme-linked immunosorbent assay (ELISA). The addition of 4-morpholinopyridine (MORP) to the SbP-SPTZ system improved its analytical parameters by decreasing the LDL of SbP to 0.03pM. These results open up very promising perspectives for using the SbP-SPTZ-MORP system in ultrasensitive immunoassay.
3-(10'-吩噻嗪基)丙烷-1-磺酸盐（SPTZ）被证明是大豆过氧化物酶（SbP）诱导化学发光的有效增强剂。据我们所知，SPTZ是首个被发现的SbP增强剂。确定了在SPTZ存在下SbP催化鲁米诺氧化的最佳条件。与传统用于化学发光酶联免疫吸附测定（ELISA）的辣根过氧化物酶-4-碘苯酚系统相比，SbP-SPTZ系统显示出更好的灵敏度和更低的检测限（LDL）。向SbP-SPTZ系统中添加4-吗啉吡啶（MORP），通过将SbP的LDL降至0.03pM，改善了其分析参数。这些结果为在超灵敏免疫测定中使用SbP-SPTZ-MORP系统开辟了非常有前景的前景。
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