Sengonul Merih, Sousa Alioscka, Libera Matthew
Department of Chemical Engineering and Materials Science, Stevens Institute of Technology, 1 Castle Point Terrace, Hoboken, NJ 07030, USA.
Colloids Surf B Biointerfaces. 2009 Oct 1;73(1):152-5. doi: 10.1016/j.colsurfb.2009.05.004. Epub 2009 May 14.
We compare the adsorption behavior of wild and surface-modified ferritin on a homopolymer blend of poly(desaminotyrosyl tyrosine dodecyl ester carbonate) (PDTD) and poly(epsilon-caprolactone) (PCL). Wild ferritin was alkylated by zero length cross-linking to surface carboxylate groups activated by water soluble carbodiimide (EDC). Modification was confirmed by anion exchange chromatography. All the adsorption experiments were done using deionized water (pH 5.8) and ultramicrotomed thin polymer films of thickness approximately 100 nm. Transmission electron microscopy shows a clear selectivity of alkylated ferritin adsorption onto the PCL phase while wild ferritin predominantly adsorbs onto the PDTD phase. We attribute these differences to the nature of the electrostatic interaction between the two types of ferritin and the polymer surface.
我们比较了野生型和表面改性铁蛋白在聚（去氨基酪氨酸十二烷基酯碳酸酯）（PDTD）和聚（ε-己内酯）（PCL）的均聚物共混物上的吸附行为。通过零长度交联将野生型铁蛋白烷基化，使其与由水溶性碳二亚胺（EDC）活化的表面羧基反应。通过阴离子交换色谱法确认了改性。所有吸附实验均使用去离子水（pH 5.8）和厚度约为100 nm的超薄切片聚合物薄膜进行。透射电子显微镜显示，烷基化铁蛋白对PCL相具有明显的吸附选择性，而野生型铁蛋白主要吸附在PDTD相上。我们将这些差异归因于两种类型的铁蛋白与聚合物表面之间静电相互作用的性质。
