[抑制ANG-2在Ishikawa细胞系中表达的作用]
[Effects of inhibition of ANG-2 expression in Ishikawa cell line].
作者信息
Zhou Huai-jun, He Rong-rong, Xia Bao-mei, Xun Qing-ying, Pan Jin-shun, Hu Ya-li
机构信息
Department of Obstetrics and Gynecology, the Affiliated Drum Tower Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu, 210008 PR China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2009 Jun;26(3):249-53. doi: 10.3760/cma.j.issn.1003-9406.2009.03.003.
OBJECTIVE
To investigate the effect of RNA interference mediated angiopoietin-2 (ANG-2) gene silencing on human endometrial carcinoma cell line Ishikawa.
METHODS
Short hairpin RNA (shRNA) targeting ANG-2 gene was designed and transfected into Ishikawa cells with Lipofectamine 2000. The mRNA and protein expression level of ANG-2, proliferation, morphological changes, apoptosis, cell cycle and invasive ability of the cells after transfection were analyzed.
RESULTS
The shRNA targeting the human ANG-2 gene effectively decreased the expression of ANG-2 on both mRNA and protein level, the proliferation inhibition rate of the Ishikawa cells was 63.11%, cell apoptosis was induced, and the cell cycle was arrested in G1 phase. The apoptotic rate of the Ishikawa cells in the transfected group was significantly higher, and the invasive ability was decreased markedly, than that of control groups.
CONCLUSION
The shRNA targeting human ANG-2 gene could reduce ANG-2 expression, inhibit cellular growth and invasion in Ishikawa cells in vitro.
目的
探讨RNA干扰介导的血管生成素-2（ANG-2）基因沉默对人子宫内膜癌细胞系Ishikawa的影响。
方法
设计靶向ANG-2基因的短发夹RNA（shRNA），并用Lipofectamine 2000转染至Ishikawa细胞中。分析转染后细胞中ANG-2的mRNA和蛋白表达水平、增殖情况、形态变化、凋亡、细胞周期及侵袭能力。
结果
靶向人ANG-2基因的shRNA有效降低了ANG-2在mRNA和蛋白水平的表达，Ishikawa细胞的增殖抑制率为63.11%，诱导了细胞凋亡，细胞周期停滞于G1期。转染组Ishikawa细胞的凋亡率显著高于对照组，侵袭能力明显降低。
结论
靶向人ANG-2基因的shRNA可降低ANG-2表达，体外抑制Ishikawa细胞的生长和侵袭。
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