Department of Pharmacology, Tehran Medical Branch, Islamic Azad University, Tehran, Iran.
Phytother Res. 2010 Feb;24(2):182-5. doi: 10.1002/ptr.2880.
To study the effectiveness of pomegranate juice on osteoarthritis, mono-iodoacetate induced loss of articular cartilage in the mouse tibiofemoral joint was used as a model. Mono-iodoacetate is an inhibitor of glycolysis which promotes osteoarthritis similar to that noted in human osteoarthritis. The histopathology of the subchondral bone and cartilage of mouse knee joints treated with a single intra-articular injection of mono-iodoacetate (0.1 mg) and killed at 1, 14 and 28 days post injection was investigated. The effect of pomegranate juice (4 mL/kg, 10 mL/kg, 20 mL/kg, orally) was studied in different groups. Histopathological changes in knee joints were seen after 2 weeks. Early osteoarthritis was characterized by areas of chondrocyte degeneration, which sometimes involved the entire thickness of the articular cartilage in the tibial plateaus and femoral condyles. Changes to the subchondral bone and proteoglycan contents, focal fragmentation and collapse of bony trabeculae with fibrosis and necrosis, and synovial cell proliferation were observed. The administration of pomegranate juice dose dependently prevented the negative effects of iodoacetate. Chondrocyte damage was significantly prevented, with proteoglycan less affected, especially in the groups receiving a high amount of pomegranate juice. No cell proliferation or inflammatory cells were detected in the synovial fluid. The effectiveness of pomegranate juice in improving histopathological damage is emphasized and its chondroprotective effect in vivo highlighted.
为了研究石榴汁对骨关节炎的疗效，我们采用了单碘乙酸诱导的小鼠胫股关节软骨丢失模型。单碘乙酸是糖酵解的抑制剂，可促进类似于人类骨关节炎的骨关节炎。研究了单次关节内注射单碘乙酸（0.1mg）后 1、14 和 28 天处死的小鼠膝关节软骨下骨和软骨的组织病理学变化，并研究了石榴汁（4ml/kg、10ml/kg、20ml/kg，口服）的作用。在不同组中观察到膝关节的组织病理学变化在 2 周后出现。早期骨关节炎的特征是软骨细胞退化区域，有时涉及胫骨平台和股骨髁的整个关节软骨厚度。观察到软骨下骨和糖胺聚糖含量的变化、骨小梁的局灶性碎裂和塌陷伴纤维化和坏死，以及滑膜细胞增殖。石榴汁的给药剂量依赖性地预防了碘乙酸的不良影响。软骨细胞损伤明显得到预防，糖胺聚糖的影响较小，尤其是在接受高剂量石榴汁的组中。滑膜液中未检测到细胞增殖或炎症细胞。强调了石榴汁改善组织病理学损伤的有效性，并突出了其体内的软骨保护作用。
