[Preparation and identification of monoclonal antibodies to staphylococcal enterotoxin I].
作者信息
Xu Jun-Ying, Ding Ding, Sun Hong-Ying, Chen Shu-Qing
机构信息
Institute of Pharmacology, Toxicology and Biological Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
出版信息
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2009 May;38(3):265-70. doi: 10.3785/j.issn.1008-9292.2009.03.008.
OBJECTIVE
To prepare and identify monoclonal antibodies against staphylococcal enterotoxin I (SEI).
METHODS
Spleen cells obtained from mice immunized with the SEI protein were fused with the myeloma cells (SP2/0). Hybridomas were screened by enzyme-linked immunosorbent assay (ELISA) and the stable monoclonal hybridomas were isolated by limiting dilution at least three times. The characters of purified monoclonal antibodies were identified by indirect ELISA and Western blotting.
RESULT
The monoclonal antibodies secreted by two hybridomas 8F7 and D8 belonged to IgG(2b) and IgG(1) subtypes. Both had high titer and specificity with no cross reaction to SEG, SEE and SEC.
CONCLUSION
The monoclonal antibodies against SEI has been successfully prepared and identified in this study.
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