Czarnowska E, Zajaczkowska A, Godlewski M M, Mroz W, Sobczak J W, Wierzchon T
Department of Pathology, The Children's Memorial Heath Institute, Al. Dzieci Polskich 20, 04-730 Warsaw, Poland.
J Nanosci Nanotechnol. 2009 Jun;9(6):3462-8. doi: 10.1166/jnn.2009.ns17.
This study is concerned with the properties and bioactivity and biocompatibility of hydroxyapatite islets deposited on a new composite layer Ti3P+Ti2Ni type produced by a duplex method on Ti6Al4V titanium alloy. The microstructure and chemical composition of a produced surface layers and hydroxyapatite coating were investigated using scanning electron microscope equipped with EDS. Their bioactivity were examined in simulated body fluid and analyzed with XPS. Dissolution of hydroxyapatite was tested in culture medium during 12 days of incubation. Biocompatibility was investigated in osteoblast Saos2 line culture in contact with the tested material. Cell proliferation and activity were determined by the MTT test and measurement of alkaline phosphatase activity, respectively. Cell distribution was analyzed under a confocal microscope. The produced surface layers have a diffusion character with fine-grained structure and about 4 microm thick external zone of Ti3P. The experiments revealed higher bioactivity and biocompatibility of the Ti3P in comparison with reference titanium alloy. Hydroxyapatite islets were 0.8 mm in diameter and about 300 nm thick. They partially dissolved during the experiment what lead to formation on Ti3P between hydroxyapatite islets a precipitate containing Ca and P. Biocompatibility analyzed under confocal microscope in range of cell adhesion with osteoblast cells of Saos2 line revealed initial the highest osteoblast adhesion on Ti3P between hydroxyapatite islets and increasing on hydroxyapatite during following days. Cell were characterized by high proliferation and ALP activity. Therefore, the high bioactivity and biocompatibility of Ti3P and profitable hydroxyapatite properties make this composite layer promising for increasing implant fixation in vivo.
本研究关注的是通过双相法在Ti6Al4V钛合金上制备的新型复合层Ti3P+Ti2Ni型上沉积的羟基磷灰石小岛的性能、生物活性和生物相容性。使用配备能谱仪的扫描电子显微镜研究了所制备的表面层和羟基磷灰石涂层的微观结构和化学成分。在模拟体液中检测了它们的生物活性，并通过X射线光电子能谱进行分析。在培养基中孵育12天期间测试了羟基磷灰石的溶解情况。在与测试材料接触的成骨细胞Saos2系培养中研究了生物相容性。分别通过MTT试验和碱性磷酸酶活性测量来确定细胞增殖和活性。在共聚焦显微镜下分析细胞分布。所制备的表面层具有扩散特征，具有细晶结构和约4微米厚的Ti3P外部区域。实验表明，与参考钛合金相比，Ti3P具有更高的生物活性和生物相容性。羟基磷灰石小岛直径为0.8毫米，约300纳米厚。在实验过程中它们部分溶解，导致在羟基磷灰石小岛之间的Ti3P上形成含有钙和磷的沉淀物。在共聚焦显微镜下分析与Saos2系成骨细胞的细胞粘附范围内的生物相容性，结果显示最初在羟基磷灰石小岛之间的Ti3P上成骨细胞粘附最高，随后几天在羟基磷灰石上增加。细胞具有高增殖和碱性磷酸酶活性。因此，Ti3P的高生物活性和生物相容性以及有利的羟基磷灰石特性使得这种复合层有望提高体内植入物的固定效果。
