Cesnjaj M, Stavljenić A, Vukicević S
Department of Anatomy, Zagreb University School of Medicine, Yugoslavia.
Acta Orthop Scand. 1991 Oct;62(5):471-5.
The effect of estrogen deficiency on matrix-induced bone formation was investigated. Female rats were ovariectomized and given demineralized bone matrix (DBM) intramuscularly 3 weeks before termination. The DBM was taken from previously ovariectomized and from sham-operated on rats. The animals were killed at various times after ovariectomy (6-27 weeks). Implants were processed undemineralized for histologic and biochemical studies. Normal DBM implanted in ovariectomized or normal rats induced extensive bone formation 6 weeks postovariectomy. The amount of newly formed bone decreased with the age of host rats. Bone matrix taken from ovariectomized rats was incompletely resorbed in both ovariectomized and normal hosts, therefore reducing the extent of osteogenesis and bone-marrow formation. Instead, chondrogenesis was intensive, but delayed. The calcium, magnesium, and zinc contents were decreased in implants taken from ovariectomized rats when compared with implants taken from normal animals. Normal osteoinduction with DBM taken from normal rats and implanted in ovariectomized rats and the absence of osteogenesis with DBM taken from ovariectomized rats indicate that an estrogen-deficient environment is not crucial for altered matrix-induced endochondral bone formation in ovariectomized rats. An altered composition of matrix from ovariectomized rats and a subsequent abnormality in the cell-matrix interaction should be considered responsible.
研究了雌激素缺乏对基质诱导骨形成的影响。对雌性大鼠进行卵巢切除，并在处死前3周肌肉注射脱矿骨基质（DBM）。DBM取自先前已切除卵巢的大鼠和假手术大鼠。在卵巢切除后的不同时间点（6 - 27周）处死动物。将植入物不脱矿进行组织学和生化研究。在卵巢切除的大鼠或正常大鼠中植入正常DBM，在卵巢切除术后6周诱导广泛的骨形成。新形成骨的量随宿主大鼠年龄的增加而减少。取自卵巢切除大鼠的骨基质在卵巢切除和正常宿主中均未完全吸收，因此降低了成骨和骨髓形成的程度。相反，软骨形成强烈，但延迟。与取自正常动物的植入物相比，取自卵巢切除大鼠的植入物中钙、镁和锌含量降低。取自正常大鼠并植入卵巢切除大鼠中的DBM能正常诱导成骨，而取自卵巢切除大鼠的DBM则无成骨作用，这表明雌激素缺乏环境对于卵巢切除大鼠中基质诱导的软骨内骨形成改变并非至关重要。应考虑取自卵巢切除大鼠的基质组成改变以及随后细胞 - 基质相互作用异常是其原因。
