Cao Yong-xiao, Long Li-hui, Ma Zhao, Tao Xiao-jun, Liu Jing, Zhou Liang-zhen
Dept. of Pharmacology, Xi'an Jiaotong University College of Medicine, Xi'an 710061, China.
Zhong Yao Cai. 2009 Feb;32(2):249-53.
To study the effect of montmorillonite on promoting diffussion of urea from blood vessel to intestine and preventing absorption of urea in intestine.
Rat intestine tract and blood vessel were perfusated circularly with intestinal tract perfusate and blood vessel perfusate with or without urea 1 g/L, respectively. The concentration of urea in perfusates was measured by urease and glutamic dehydrogenase coupling method.
The blood vessel was circularly perfusated with vascular perfusate containing urea and intestinal tract was circularly perfusated with intestinal perfusate without urea. The concentration of urea in vascular perfusate decreased gradually, and the concentration of urea in intestine perfusate increased slowly. When montmorillonite was added into the intestinal tract, the urea concentration in both intestinal tract perfusate and the vascular perfusate in montmorillonite 0.5 g/kg and 1.0 g/kg groups were significantly lower than that of control group (P < 0.05). The blood vessel was circularly perfusated with vascular perfusate without urea and intestinal tract was circularly perfusated with perfusate containing urea, the concentration of urea in intestine perfusate decreased little by little, and the concentration of urea in vascular perfusate increased slowly. After montmorillonite was administrated into the intestinal tract, the urea concentrations in both the vascular perfusate and intestinal tract perfusate in montmorillonite 0.5 g/kg and 1.0 g/kg were obviously lower than that of control group (P < 0.05).
Montmorillonite promotes the diffusion of urea from blood vessel to intestine, and inhibits the absorption of urea in intestine.
研究蒙脱石对促进尿素从血管向肠道扩散及阻止肠道对尿素吸收的作用。
分别用含或不含1 g/L尿素的肠道灌流液和血管灌流液对大鼠肠道和血管进行循环灌流。采用脲酶和谷氨酸脱氢酶偶联法测定灌流液中尿素浓度。
血管用含尿素的血管灌流液进行循环灌流，肠道用不含尿素的肠道灌流液进行循环灌流，血管灌流液中尿素浓度逐渐降低，肠道灌流液中尿素浓度缓慢升高。当向肠道中加入蒙脱石时，蒙脱石0.5 g/kg和1.0 g/kg组的肠道灌流液和血管灌流液中尿素浓度均显著低于对照组（P<0.05）。血管用不含尿素的血管灌流液进行循环灌流，肠道用含尿素的灌流液进行循环灌流，肠道灌流液中尿素浓度逐渐降低，血管灌流液中尿素浓度缓慢升高。向肠道给予蒙脱石后，蒙脱石0.5 g/kg和1.0 g/kg组的血管灌流液和肠道灌流液中尿素浓度均明显低于对照组（P<0.05）。
蒙脱石促进尿素从血管向肠道扩散，并抑制肠道对尿素的吸收。
