[The morphometric analysis of cytotoxical action of rats and mice splenocytes against confluent monolayer cell lines of hepatomas].

The present study was aimed to examine the possibility to use of the morphometric analysis for estimation of the total natural cytotoxic activity of rat and mice C3HA splenocytes against the cultured target cells which formed confluent monolayers. By means of this method we have revealed that two rat monolayer hepatoma cell lines -- HTC and Zajdela -- were sensitive to cytolysis mediated by rat effector cells, but not splenocytes of C3HA mice. The mild pretreatment with 0.5% paraformaldehyde of the rat splenocytes produced a significant decrease only in the cytotoxic activity against the target cells line Zajdela and don't affect the lysis of the other target HTC cells. These results suggest that the natural cytotoxic rat cells may mediate their lytic functions toward tested target lines by different ways. In the case of HTC cells as the targets the effectors induce death receptor-mediated apoptosis, as to target cells Zajdela they deliver lethal hit by perforin-granzyme exocytosis mechanism. The cultured cell monolayers of mice hepatoma -- MH-22a and BWTG3 -- cells showed under conditions of our experiments the resistance to cytolysis by homological effector cells; however, the hepatoma MH-22a cells were susceptible to killing mediated by rat cytotoxic splenocytes.