Voronin D A, Bochernikov A M, Baricheva E M, Zakharov I K, Kiseleva E V
Tsitologiia. 2009;51(4):335-45.
Comparative analyses of symbiotic bacteria Wolbachia (stamm wMelPop reducing lifespan of flies) morphology in normal and mutant strains of Drosophila melanogaster as well as the influence of Wolbachia on the host cell ultrastructure have been done. Wolbachia infected D. melanogaster mutation strains Trithorax-like -- Trl362/TM3, Sb1 Ser y+ and Trlen82/TM3, Sb1 Ser y+ have been received by special flies crossing. Uninfected strain D. melanogaster white-1118 (w1118) have been obtained by antibiotic treatment of initially infected strain D. melanogaster [w]w1118. Complex of different methods and approaches let to investigate for the first time the morphology of cell structure before and after bacterial infection of insects and to value the bacterial presence effect on flies viability and reproduction of normal and mutant flies. Morphology af cytoplasmic compartments in early embryos and eggs layed by infected and uninfecyed females Trl362/TM3, Sb1 Ser y+ and Trlen82/TM3, Sb1 Ser y+ have been analyzed. Electron microscopy has shown that D. melanogaster embryos contain typical Wolbachia contacting with different host organelles that verify preservation of their functional activity. Atificial mitochondria and Wolbachia (wMelPop) of unusual morphology with defective bacterial membranes have been visualised in D. melanogaster [w]Trl362/TM3, Sb1 Ser y+. Wolbachia presence in ovarium cells from strains [w]Trl362/TM3, Sb1 Ser y+ and [w]Trlen82/TM3, Sb1 Ser y+ did not influence on eggs quantity layed by females. We have demonstrated for the first time that lifespan of infected and uninfected strains: D. melanogaster Trl362/TM3, Sb1 Ser y+ and Trlen82/TM3, Sb1 Ser y+ were similar. However the lifespan of imago from strain [w]w1118 was lower in comparison to those from strains Trl362/TM3, Sb1 Ser y+ and Trlen82/TM3, Sb1 Ser y+. It suggests that either chromosomal balancer TM3 or Trl mutation play an importance role in host-symbiotic relationship. Next experiments have revealed that lifespan of homozygotic flies decreased essentially and was close to lifespan of strain [w]w1118. Data obtained confirm that chromosomal balancer TM3 can affect on symbiont-host relationship.
已对共生细菌沃尔巴克氏体（wMelPop株系可缩短果蝇寿命）在正常和突变型黑腹果蝇品系中的形态进行了比较分析，同时也研究了沃尔巴克氏体对宿主细胞超微结构的影响。通过特殊的果蝇杂交获得了感染沃尔巴克氏体的黑腹果蝇突变株系Trithorax样——Trl362/TM3，Sb1 Ser y +和Trlen82/TM3，Sb1 Ser y +。未感染的黑腹果蝇品系white - 1118（w1118）是通过对最初感染的黑腹果蝇品系[w]w1118进行抗生素处理获得的。多种不同方法和途径的综合运用，首次使得研究昆虫细菌感染前后细胞结构的形态成为可能，并评估了细菌存在对正常和突变果蝇的生存能力及繁殖的影响。已分析了感染和未感染的Trl362/TM3，Sb1 Ser y +和Trlen82/TM3，Sb1 Ser y +雌蝇所产早期胚胎和卵中细胞质区室的形态。电子显微镜显示，黑腹果蝇胚胎中含有典型的与不同宿主细胞器接触的沃尔巴克氏体，这证实了其功能活性的保持。在黑腹果蝇[w]Trl362/TM3，Sb1 Ser y +中观察到了形态异常且细菌膜有缺陷的人工线粒体和沃尔巴克氏体（wMelPop）。来自[w]Trl362/TM3，Sb1 Ser y +和[w]Trlen82/TM3，Sb1 Ser y +品系的卵巢细胞中存在沃尔巴克氏体，这对雌蝇产卵数量没有影响。我们首次证明，感染和未感染的品系：黑腹果蝇Trl362/TM3，Sb1 Ser y +和Trlen82/TM3，Sb1 Ser y +的寿命相似。然而，与Trl362/TM3，Sb1 Ser y +和Trlen82/TM3，Sb1 Ser y +品系相比，[w]w1118品系成虫的寿命较短。这表明染色体平衡子TM3或Trl突变在宿主 - 共生关系中起重要作用。接下来的实验表明，纯合果蝇的寿命显著缩短，接近[w]w1118品系的寿命。获得的数据证实，染色体平衡子TM3会影响共生体与宿主的关系。
