Ruijter J M, Baker R E, De Jong B M, Romijn H J
The Netherlands Institute for Brain Research, Amsterdam.
Int J Dev Neurosci. 1991;9(4):331-8. doi: 10.1016/0736-5748(91)90054-p.
Culture thickness, numerical density of neurons and neuronal survival were studied in timed series of control and tetrodotoxin-silenced neocortical cultures to provide information on the role of bioelectric activity on neuronal development. In control cultures, culture thickness and number of surviving neurons decrease during the first weeks in vitro, but remain constant between 2 and 3 weeks indicating that the cultures are essentially mature. In the 4th week in vitro a further decrease in surviving neurons was observed. In tetrodotoxin-treated cultures the number of surviving neurons decreased significantly between 1 and 2 weeks in vitro, to remain constant thereafter. However, culture thickness significantly increased at 3 and 4 weeks in vitro after an initial drop between 1 and 2 weeks. Compared to age-matched controls at 2 and 3 weeks in vitro, only ca 50% of the neurons survived the loss of bioelectric activity. Similar differences were present between 1 and 2 weeks. Thus, the loss of all measurable bioelectric activity induces neuronal death in neocortical explants, but promotes neuropil formation by the surviving cells.
在对照和经河豚毒素沉默的新皮质培养物的定时系列中，研究了培养物厚度、神经元的数量密度和神经元存活情况，以提供有关生物电活动对神经元发育作用的信息。在对照培养物中，培养物厚度和存活神经元的数量在体外培养的最初几周内减少，但在2至3周之间保持恒定，这表明培养物基本成熟。在体外培养的第4周，观察到存活神经元进一步减少。在经河豚毒素处理的培养物中，存活神经元的数量在体外培养1至2周之间显著减少，此后保持恒定。然而，在1至2周之间最初下降之后，培养物厚度在体外培养3周和4周时显著增加。与体外培养2周和3周时年龄匹配的对照相比，只有约50%的神经元在生物电活动丧失后存活下来。在1至2周之间也存在类似差异。因此，所有可测量的生物电活动丧失会诱导新皮质外植体中的神经元死亡，但会促进存活细胞的神经纤维网形成。
