Wang Peizheng, Su Li, Qin Li, Hu Baomin, Guo Wangzhen, Zhang Tianzhen
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, China.
Theor Appl Genet. 2009 Aug;119(4):733-9. doi: 10.1007/s00122-009-1084-4. Epub 2009 Jun 9.
Fusarium wilt (FW) is one of the most economically damaging cotton diseases worldwide, causing yellowing, wilting, defoliation, vascular tissue damage and ultimately death. Identification of molecular markers linked to FW genes is vital to incorporate resistance into elite cotton cultivars. An intraspecific F(2) in Gossypium hirsutum L. was developed by crossing with a highly resistant cultivar Zhongmiansuo 35 (ZMS35) and a susceptible cultivar Junmian 1 to screen simple sequence repeats (SSRs) closely linked to the FW resistance gene. FW was identified in F(2:3) families by evaluating seedling leaf symptoms and vascular tissue damage at plant maturity under natural field infection conditions over 2 years. The results showed that FW resistance segregated in a 3:1 ratio as a simple monogenic trait in F(2:3) families. Molecular mapping identified a FW resistance gene closely linked with the SSR marker JESPR304(-280) in chromosome D3(c17). We proposed to name this gene FW ( R ). A composite interval mapping method detected four QTLs for FW resistance in Chr.A7(c7), D1(c15), D9(c23) and D3, respectively. Among them, one major QTL (LOD > 20) was tagged near marker JESPR304 within an interval of 0.06-0.2 cM, and explained over 52.5-60.9% of the total phenotypic variance. The data confirmed the existence of a major gene in Chr.D3. This is the first report of molecular mapping of a major gene contributing FW resistance in cotton. The present research therefore provides an opportunity to understand the genetic control of resistance to FW and conduct molecular marker-assisted selection breeding to develop FW resistant cultivars.
枯萎病（FW）是全球对棉花经济危害最大的病害之一，会导致棉花发黄、枯萎、落叶、维管束组织受损，最终死亡。鉴定与FW基因连锁的分子标记对于将抗性导入优良棉花品种至关重要。通过将高抗品种中棉所35（ZMS35）与感病品种军棉1杂交，构建了陆地棉种内F₂群体，以筛选与FW抗性基因紧密连锁的简单序列重复（SSR）标记。在两年的自然田间感染条件下，通过评估F₂:₃家系幼苗叶片症状和植株成熟时维管束组织损伤来鉴定FW。结果表明，在F₂:₃家系中，FW抗性以简单单基因性状的形式按3:1的比例分离。分子图谱分析确定了一个与染色体D3（c₁₇）上的SSR标记JESPR304（-280）紧密连锁的FW抗性基因。我们建议将该基因命名为FW（R）。复合区间作图法分别在Chr.A7（c₇）、D1（c₁₅）、D9（c₂₃）和D3中检测到4个FW抗性QTL。其中，一个主效QTL（LOD>20）在标记JESPR304附近，区间为0.06 - 0.2 cM，解释了超过52.5 - 60.9%的总表型变异。数据证实了Chr.D3中存在一个主基因。这是棉花中一个对FW抗性起作用的主基因分子图谱分析的首次报道。因此，本研究为了解棉花对FW抗性的遗传控制以及进行分子标记辅助选择育种以培育抗FW品种提供了契机。
