[SRM5/CDC28、SRM8/NET1和SRM12/HFI1基因在酿酒酵母细胞周期检验点控制中的作用]

[Participation of SRM5/CDC28, SRM8/NET1, and SRM12/HFI1 genes in checkpoint control in yeast Saccharomyces cerevisiae].

作者信息

Kadyshevskaia E Iu, Koltovaia N A

出版信息

Genetika. 2009 Apr;45(4):458-70.

PMID:19507699

Abstract

About twenty genes participating in checkpoint control are known in yeast Saccharomyces cerevisiae. The involvement of SRM genes in the cell cycle arrest under the action of injurious agents was studied in this work. These genes were earlier defined as genes affecting genetic stability and radiosensitivity. It was shown that mutations srm5/cdc28-srm, srm8/net1-srm, and srm12/hfi1-srm shorten the cell cycle arrest in the presence of DNA damage and influence the checkpoint arrest in G0/S (srm5, srm8), G1/S (srm5, srm8, srm12), S (srm5, srm12), and G2/M (srm5). It seems likely that genes SRM5/CDC28, SRM12/HFI1/ADA1, and SRM8/NET1 are involved in the formation of a cell response to DNA damage, and in checkpoint regulation in particular.

摘要

在酿酒酵母中已知约有二十个参与检查点控制的基因。在这项研究中，对SRM基因在损伤因子作用下细胞周期停滞中的作用进行了研究。这些基因先前被定义为影响遗传稳定性和辐射敏感性的基因。结果表明，突变体srm5/cdc28-srm、srm8/net1-srm和srm12/hfi1-srm在存在DNA损伤的情况下会缩短细胞周期停滞时间，并影响G0/S（srm5、srm8）、G1/S（srm5、srm8、srm12）、S（srm5、srm12）和G2/M（srm5）期的检查点停滞。SRM5/CDC28、SRM12/HFI1/ADA1和SRM8/NET1基因似乎参与了细胞对DNA损伤反应的形成，尤其是在检查点调节方面。