基于45S rDNA荧光原位杂交和5S rDNA序列对紫苏五个变种的比较分析
Comparative analysis of five varieties in Perilla frutescens (L.) Britton by 45S rDNA FISH and 5S rDNA sequences.
作者信息
Diao Y, Miao Y, Lin X, Liao C, Guo F, Hu Z
机构信息
Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Science, Wuhan University, Wuhan 430072, China.
出版信息
Genetika. 2009 Apr;45(4):506-10.
In this study, the relationships of five varieties in Perilla frutescens (L.) Britton were analyzed. All the varieties showed the same chromosome number 2n = 40, and the banding patterns of PI-DAPI staining and the FISH results of 45S rDNA probe were on the same site of the chromosome pair. The 5S rRNA gene spacers were ranged from 413 bp in P. frutescens var. purpurascens and 408 bp in var. frutescens. The varieties analyzed here were clustered into two clades according to the phylogenetic analysis. The sequence identity of 96% between var. auriculato-dentata and var. arguta suggested they could be considered the same variety.
本研究分析了紫苏（Perilla frutescens (L.) Britton）五个变种之间的关系。所有变种的染色体数目均相同，为2n = 40，PI-DAPI染色的带型模式以及45S rDNA探针的荧光原位杂交结果均位于染色体对的同一位置。5S rRNA基因间隔区在紫苏变种紫背紫苏中为413 bp，在紫苏变种中为408 bp。根据系统发育分析，这里分析的变种被聚类为两个分支。耳齿变种和白苏变种之间96%的序列同一性表明它们可被视为同一变种。
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