Sakemi F, Nakayasu K, Okisaka S
Department of Ophthalmology, National Defense Medical College, Tokorozawa-shi, Japan.
Nippon Ganka Gakkai Zasshi. 1991 Aug;95(8):721-7.
It is difficult to maintain corneal epithelial cells for several passages in culture. At a first step to establish a culture system for corneal epithelium, the authors evaluated factors which seemed to be involved in the growth kinetics and mitotic activity of rabbit corneal epithelium in vitro. First of all, to compare the growth kinetics of peripheral and central epithelium, we measured the areas and distances of the epithelium that outgrew from peripheral and central explants in culture. The peripheral corneal epithelium grew 1.2-to 1.6-fold better than the central corneal epithelium. However, there was no significant difference between them. Secondly, we evaluated the effects of epidermal growth factor (EGF), cholera toxin (CTX) and insulin, which were contained in supplemented hormonal epithelial medium (SHEM) developed by Jumblatt et al, on the mitotic activity of rabbit corneal epithelium. Among these drugs, only EGF had the ability to increase the epithelial cell number after 10 days of incubation. By contrast, CTX which elevated the level of cyclic AMP inhibited the proliferation of the epithelium.
在培养中维持角膜上皮细胞传代培养数代是困难的。在建立角膜上皮细胞培养系统的第一步，作者评估了一些似乎与兔角膜上皮细胞体外生长动力学和有丝分裂活性有关的因素。首先，为了比较周边和中央上皮细胞的生长动力学，我们测量了培养中外植体周边和中央生长出的上皮细胞的面积和距离。周边角膜上皮细胞的生长比中央角膜上皮细胞好1.2至1.6倍。然而，它们之间没有显著差异。其次，我们评估了Jumblatt等人开发的补充激素上皮培养基（SHEM）中所含的表皮生长因子（EGF）、霍乱毒素（CTX）和胰岛素对兔角膜上皮细胞有丝分裂活性的影响。在这些药物中，只有EGF在孵育10天后有增加上皮细胞数量的能力。相比之下，提高环磷酸腺苷水平的CTX抑制了上皮细胞的增殖。
