Zhang Yuan, Wang Xiaobei, Ma Ling, Wang Zehua, Hu Lihua
Department of Gynaecology and Obstetrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2009 Jun;29(3):368-71. doi: 10.1007/s11596-009-0321-z. Epub 2009 Jun 10.
This study evaluated the clinical significance of hTERC gene amplification detection by fluorescence in situ hybridization (FISH) in the screening of cervical lesions. Cervical specimens of 50 high risk patients were detected by thin liquid-based cytology. The patients whose cytological results were classified as ASCUS or above were subjected to the subsequent colposcopic biopsies. Slides prepared from these 50 cervical specimens were analyzed for hTERC gene amplification using interphase FISH with the two-color hTERC probe. The results of the cytological analysis and those of subsequent biopsies, when available, were compared with the FISH-detected hTERC abnormalities. It was found that the positive rates of hTERC gene amplification in NILM, ASCUS, LSIL, HSIL, and SCC groups were 0.00, 28.57%, 57.14%, 100%, and 100%, respectively. The positive rates of hTERC gene amplification in HSIL and SCC groups were significantly higher than those in NILM, ASCUS and LSIL groups (all P<0.05). The mean percentages of cells with hTERC gene amplification in NILM, ASCUS, LSIL, HSIL, and SCC groups were 0.00, 10.50%, 36.00%, 79.00%, and 96.50%, respectively. Patients with HSIL or SCC cytological diagnoses had significantly higher mean percentages of cells with hTERC gene amplification than did patients with NILM, ASCUS or LSIL cytological diagnoses (all P<0.05). It was concluded that two-color interphase FISH could detect hTERC gene amplification to accurately distinguish HSIL and ISIL of cervical cells. It may be an adjunct to cytology screening, especially high-risk patients.
本研究评估了荧光原位杂交（FISH）检测hTERC基因扩增在宫颈病变筛查中的临床意义。对50例高危患者的宫颈标本进行薄层液基细胞学检测。将细胞学结果分类为非典型鳞状细胞不能明确意义（ASCUS）及以上的患者进行后续阴道镜活检。使用双色hTERC探针通过间期FISH分析从这50例宫颈标本制备的玻片，以检测hTERC基因扩增。将细胞学分析结果以及后续活检结果（若有）与FISH检测到的hTERC异常情况进行比较。结果发现，在正常宫颈上皮细胞（NILM）、ASCUS、低度鳞状上皮内病变（LSIL）、高度鳞状上皮内病变（HSIL）和鳞状细胞癌（SCC）组中，hTERC基因扩增的阳性率分别为0.00%、28.57%、57.14%、100%和100%。HSIL和SCC组中hTERC基因扩增的阳性率显著高于NILM、ASCUS和LSIL组（均P<0.05）。NILM、ASCUS、LSIL、HSIL和SCC组中具有hTERC基因扩增的细胞的平均百分比分别为0.00%、10.50%、36.00%、79.00%和96.50%。细胞学诊断为HSIL或SCC的患者具有hTERC基因扩增的细胞的平均百分比显著高于细胞学诊断为NILM、ASCUS或LSIL的患者（均P<0.05）。结论是，双色间期FISH可检测hTERC基因扩增以准确区分宫颈细胞的HSIL和LSIL。它可能是细胞学筛查的辅助手段，尤其是高危患者。
