[不同比例的冷冻保护剂与精浆对解冻后人类精子活力的影响]

[Effects of different proportions of cryoprotectant to seminal plasma on the motility of post-thaw human sperm].

作者信息

Hu Jing-Mei, Xu Cheng-Yan, Li Yuan, Geng Ling, Ma Gang, Wang Li, Chen Zi-Jiang

机构信息

Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong University, Jinan, Shandong 250021, China.

出版信息

Zhonghua Nan Ke Xue. 2009 May;15(5):422-4.

PMID:19514554

Abstract

OBJECTIVE

To investigate the effects of different proportions of cryoprotectant to seminal plasma on the motility of post-thaw human sperm.

METHODS

Different proportions of cryoprotectant to seminal plasma (1:1 and 1:3) were used for freezing sperm, and the forward movement and total motility rates of the frozen-thawed sperm were compared.

RESULTS

The forward movement and total motility rates were (58.60 +/- 5.57)% and (66.17 +/- 5.24)% before cryopreservation. The 1:1 proportion achieved post-thaw forward movement and total motility rates of (40.53 +/- 8.97)% and (51.23 +/- 9. 30)%, while the 1:3 (44.7 +/- 8.67)% and (51.50 +/- 7.40)%, respectively. Significantly decreased sperm motility was observed after cryopreservation (P < 0.05). Statistically significant differences were found in the forward movement but not in the total motility of the frozen-thawed sperm between the two proportions.

CONCLUSION

Cryopreservation causes obvious damage to human sperm. Higher proportion of cryoprotectant to seminal plasma (1:3) can improve the forward movement of post-thaw sperm as compared with the lower one (1:1).

摘要

目的

探讨不同比例的冷冻保护剂与精浆对解冻后人类精子活力的影响。

方法

采用不同比例的冷冻保护剂与精浆（1:1和1:3）冷冻精子，比较冻融后精子的前向运动率和总活力率。

结果

冷冻保存前精子的前向运动率和总活力率分别为（58.60±5.57）%和（66.17±5.24）%。1:1比例的冻融后精子前向运动率和总活力率分别为（40.53±8.97）%和（51.23±9.30）%，而1:3比例的分别为（44.7±8.67）%和（51.50±7.40）%。冷冻保存后精子活力显著下降（P<0.05）。两种比例的冻融后精子在前向运动方面存在统计学显著差异，但在总活力方面无差异。