Roberton A M, Rabel B, Harding C A, Tasman-Jones C, Harris P J, Lee S P
Department of Biochemistry, University of Auckland, New Zealand.
Am J Physiol. 1991 Nov;261(5 Pt 1):G728-34. doi: 10.1152/ajpgi.1991.261.5.G728.
To establish a human model system for investigating control of mucus secretion by the small intestine, we have studied the output of mucus glycoprotein in the urine of patients with ileal conduits. A highly specific enzyme-linked immunosorbent assay (ELISA) was used to measure mucus glycoprotein with a sensitivity down to 0.3 ng protein of mucus glycoprotein. The assay detects human ileal mucus glycoprotein in the urine of ileal conduit patients; for an individual subject, the amount hardly varied from day to day, although there were substantial differences between individuals in the amounts secreted. Control urine from persons with normal bladders did not contain any mucus glycoprotein that reacted in the ELISA. The model has many advantages over other experimental systems for studying the rate of mucus glycoprotein secretion from the human ileum and can also be used as a reproducible resource of undegraded mucus glycoprotein.
为建立一个用于研究小肠黏液分泌调控的人体模型系统，我们研究了回肠造口术患者尿液中黏液糖蛋白的排出情况。采用一种高度特异性的酶联免疫吸附测定法（ELISA）来检测黏液糖蛋白，其灵敏度低至0.3纳克黏液糖蛋白蛋白。该测定法可检测回肠造口术患者尿液中的人回肠黏液糖蛋白；对于个体而言，尽管个体之间分泌量存在显著差异，但每天的分泌量几乎没有变化。膀胱正常者的对照尿液在ELISA中未检测到任何有反应的黏液糖蛋白。与其他用于研究人回肠黏液糖蛋白分泌速率的实验系统相比，该模型具有许多优势，并且还可作为未降解黏液糖蛋白的可重复资源。
