Charradi Khaled, Forano Claude, Prevot Vanessa, Ben Haj Amara Abdesslem, Mousty Christine
Departement de Chimie Moleculaire (DCM, UMR CNRS-UJF 5250) Universite Joseph Fourier, 38041 Grenoble, France.
Langmuir. 2009 Sep 1;25(17):10376-83. doi: 10.1021/la901080r.
The possible role of structural iron in clays to promote direct electron transfer of hemoglobin (Hb) was investigated. Clays containing different amounts of iron situated in octahedral or tetrahedral sites have been used to modify glassy carbon electrodes: nontronite, synthetic montmorillonite, and saponite. A synthetic montmorillonite containing non-iron impurities was used as a reference. Interactions between Hb and these clays were studied with the establishment of adsorption isotherms and by the analysis of X-ray diffraction patterns, FTIR, and UV-vis spectra of the Hb-clay samples. The electrochemical behavior of clay modified electrodes (CME) was characterized by cyclic voltammetry in the presence of Hb in solution or adsorbed within the clays. Nontronite, which contains the highest amount of structural iron, enhanced significantly direct electron transfer of Hb. Finally, the electrocatalytic behavior of Hb-Nontronite CME in the presence of hydrogen peroxide was also studied, and the H(2)O(2) calibration curve was recorded under amperometric conditions for different bioelectrode configurations.
研究了粘土中结构铁促进血红蛋白（Hb）直接电子转移的可能作用。含有不同量位于八面体或四面体位置的铁的粘土已被用于修饰玻碳电极：绿脱石、合成蒙脱石和皂石。使用含有非铁杂质的合成蒙脱石作为参考。通过建立吸附等温线以及分析Hb-粘土样品的X射线衍射图谱、傅里叶变换红外光谱（FTIR）和紫外可见光谱，研究了Hb与这些粘土之间的相互作用。通过在溶液中或吸附在粘土中的Hb存在下进行循环伏安法，表征了粘土修饰电极（CME）的电化学行为。含有最高量结构铁的绿脱石显著增强了Hb的直接电子转移。最后，还研究了Hb-绿脱石CME在过氧化氢存在下的电催化行为，并在不同生物电极配置的安培条件下记录了H₂O₂校准曲线。
