通过氟碳萃取法纯化疱疹病毒核衣壳。
Purification of herpesvirus nucleocapsids by fluorocarbon extraction.
作者信息
Sottong P, Harris R, Graham B, Rupp B, Bell C, Conlon T, Klecker J
出版信息
Microbios. 1976;16(64):105-10.
PMID:195183
Abstract
Purified nucleocapsids were prepared from herpes simplex virus type-1 and herpes simplex virus type-2 infected cells, using a fluorocarbon extraction technique with Freon 113. Fifty to 85% of the total nucleocapsid population remained 'nucleated' or full in terms of the nucleic acid core making them useful as a source of DNA. CsCl banded DNA represented 30--40% of the theoretical yield of the total nucleocapsid population.
摘要
使用氟利昂113的氟碳萃取技术，从感染单纯疱疹病毒1型和单纯疱疹病毒2型的细胞中制备纯化的核衣壳。就核酸核心而言，50%至85%的核衣壳群体保持“有核”或完整状态，这使得它们成为有用的DNA来源。氯化铯梯度离心得到的DNA占核衣壳群体理论产量的30%至40%。
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