Kopecká Marie, Gabriel Miroslav
Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Chemotherapy. 2009;55(4):278-86. doi: 10.1159/000224655. Epub 2009 Jun 10.
The cytoskeleton was investigated as a potential target for the inhibition of cell division in Fellomyces fuzhouensis CBS 8243 related to Cryptococcus neoformans.
Vincristine, vinblastine, paclitaxel, methyl benzimidazole-2-yl carbamate (BCM), thiabendazole, cytochalasins A, B and D and latrunculin A were added to yeast extract peptone dextrose medium containing cells, investigated by phase contrast and fluorescence microscopy, counted in a Burker chamber and absorbance was measured.
Vincristine, vinblastine, paclitaxel, cytochalasins A, B and D transiently blocked proliferation. BCM disrupted microtubules and inhibited mitosis, but F-actin patches and cables persisted and neck-less conidia appeared without stalks. Latrunculin disrupted F-actin, cells became spherical, and stalks and necks degenerated; microtubules persisted, but mitosis, cytokinesis and conidiogenesis were blocked. The combined application of latrunculin and BCM disrupted F-actin and microtubules, and inhibited cells became spherical and did not divide.
Microtubules and F-actin are effective targets for permanent inhibition of nuclear and cell division and conidiogenesis by BCM and latrunculin A.
细胞骨架被作为与新型隐球菌相关的福州费洛蒙酵母CBS 8243中细胞分裂抑制的潜在靶点进行研究。
将长春新碱、长春碱、紫杉醇、甲基苯并咪唑-2-基氨基甲酸酯（BCM）、噻苯达唑、细胞松弛素A、B和D以及拉春库林A添加到含有细胞的酵母提取物蛋白胨葡萄糖培养基中，通过相差显微镜和荧光显微镜进行观察，在血细胞计数板中计数并测量吸光度。
长春新碱、长春碱、紫杉醇、细胞松弛素A、B和D可短暂阻断增殖。BCM破坏微管并抑制有丝分裂，但F-肌动蛋白斑块和束状结构持续存在，且出现无柄的无颈分生孢子。拉春库林破坏F-肌动蛋白，细胞变成球形，柄和颈部退化；微管持续存在，但有丝分裂、胞质分裂和分生孢子形成被阻断。拉春库林和BCM联合应用破坏F-肌动蛋白和微管，抑制细胞变成球形且不分裂。
微管和F-肌动蛋白是BCM和拉春库林A永久抑制核分裂、细胞分裂和分生孢子形成的有效靶点。
