使用未修饰的金纳米颗粒作为比色探针进行特定DNA序列——端粒的无标记检测。
Label-free detection of specific DNA sequence-telomere using unmodified gold nanoparticles as colorimetric probes.
作者信息
Qi Yingying, Li Li, Li Baoxin
机构信息
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2009 Sep 15;74(1):127-31. doi: 10.1016/j.saa.2009.05.023. Epub 2009 May 23.
A simple and sensitive label-free colorimetric detection of telomere DNA has been developed. It was based on the color change of gold nanoparticles (AuNPs) due to DNA hybridization. UV-vis spectra and transmission electron microscopy (TEM) were used to investigate the change of AuNPs. Under the optimized conditions, the linear range for determination of telomere DNA was 5.7 x 10(-13) to 4.5 x 10(-6)mol/L. The detection limit (3 sigma) of this method has decreased to pico-molar level.
一种简单且灵敏的无标记比色法检测端粒DNA的方法已被开发出来。它基于DNA杂交导致的金纳米颗粒（AuNPs）颜色变化。利用紫外可见光谱和透射电子显微镜（TEM）研究AuNPs的变化。在优化条件下，端粒DNA测定的线性范围为5.7×10⁻¹³至4.5×10⁻⁶mol/L。该方法的检测限（3σ）已降至皮摩尔水平。
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