Luo Hongchun, Zhang Hongbin, Zhang Zhenzhen, Zhang Xia, Ning Bo, Guo Jinjun, Nie Na, Liu Bo, Wu Xiaoling
Department of Gastroenterology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, PR China.
J Exp Clin Cancer Res. 2009 Jun 16;28(1):82. doi: 10.1186/1756-9966-28-82.
MircoRNAs(miRNAs) are short, endogenously non-coding RNAs. The abnormal expression of miRNAs may be valuable for the diagnosis and treatment of tumors.
To screening the special miRNAs in gastric carcinoma, expression level of miRNAs in gastric carcinoma and normal gaster samples were detected by miRNA gene chip. Then, the expressions of miR-9 and miR-433 in gastric carcinoma tissue and SGC7901 cell line were validated by qRT-PCR. GRB2 and RAB34, targets of miR-433 and miR-9 respectively, were detected by Western blot.
We found 19 miRNAs and 7 miRNAs were down-regulated and up-regulated respectively. Compared with normal gaster samples, our data showed that miR-9 and miR-433 were down-regulated in gastric carcinoma. Meanwhile, we also found that miR-433 and miR-9 regulated the expression levels of GRB2 and RAB34 respectively.
Our data show miR-9 and miR-433 was down-regulated in gastric carcinoma. The targets of miR-433 and miR-9 were tumor-associated proteins GRB2 and RAB34 respectively. This result provided the related information of miRNAs in gastric carcinoma.
微小RNA（miRNA）是短链内源性非编码RNA。miRNA的异常表达可能对肿瘤的诊断和治疗具有重要价值。
为筛选胃癌中的特异性miRNA，采用miRNA基因芯片检测胃癌组织和正常胃组织样本中miRNA的表达水平。然后，通过qRT-PCR验证miR-9和miR-433在胃癌组织和SGC7901细胞系中的表达。分别通过蛋白质免疫印迹法检测miR-433和miR-9的靶标GRB2和RAB34。
我们发现分别有19个miRNA表达下调，7个miRNA表达上调。与正常胃组织样本相比，我们的数据显示miR-9和miR-433在胃癌中表达下调。同时，我们还发现miR-433和miR-9分别调节GRB2和RAB34的表达水平。
我们的数据表明miR-9和miR-433在胃癌中表达下调。miR-
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