结直肠微小RNA组
The colorectal microRNAome.
作者信息
Cummins Jordan M, He Yiping, Leary Rebecca J, Pagliarini Ray, Diaz Luis A, Sjoblom Tobias, Barad Omer, Bentwich Zvi, Szafranska Anna E, Labourier Emmanuel, Raymond Christopher K, Roberts Brian S, Juhl Hartmut, Kinzler Kenneth W, Vogelstein Bert, Velculescu Victor E
机构信息
The Sidney Kimmel Comprehensive Cancer Center and Howard Hughes Medical Institute, Johns Hopkins University Medical Institutions, Baltimore, MD 21231, USA.
出版信息
Proc Natl Acad Sci U S A. 2006 Mar 7;103(10):3687-92. doi: 10.1073/pnas.0511155103. Epub 2006 Feb 27.
MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. The public miRNA database contains 321 human miRNA sequences, 234 of which have been experimentally verified. To explore the possibility that additional miRNAs are present in the human genome, we have developed an experimental approach called miRNA serial analysis of gene expression (miRAGE) and used it to perform the largest experimental analysis of human miRNAs to date. Sequence analysis of 273,966 small RNA tags from human colorectal cells allowed us to identify 200 known mature miRNAs, 133 novel miRNA candidates, and 112 previously uncharacterized miRNA* forms. To aid in the evaluation of candidate miRNAs, we disrupted the Dicer locus in three human colorectal cancer cell lines and examined known and novel miRNAs in these cells. These studies suggest that the human genome contains many more miRNAs than currently identified and provide an approach for the large-scale experimental cloning of novel human miRNAs in human tissues.
微小RNA（miRNA）是一类小的非编码RNA，在多细胞生物中具有重要的调控作用。公共miRNA数据库包含321条人类miRNA序列，其中234条已通过实验验证。为了探索人类基因组中是否存在其他miRNA，我们开发了一种名为miRNA基因表达序列分析（miRAGE）的实验方法，并使用它进行了迄今为止最大规模的人类miRNA实验分析。对来自人类结肠直肠细胞的273966个小RNA标签进行序列分析，使我们能够鉴定出200个已知的成熟miRNA、133个新的miRNA候选物以及112种以前未鉴定的miRNA*形式。为了帮助评估候选miRNA，我们在三种人类结肠直肠癌细胞系中破坏了Dicer基因座，并检测了这些细胞中的已知和新miRNA。这些研究表明，人类基因组中含有的miRNA比目前已鉴定的要多得多，并为在人类组织中大规模实验性克隆新的人类miRNA提供了一种方法。
Zotero 插件