Dietzsch E, Hong J, Leslie D E, Martin L, Rolland J, Benson E, Toh B H, McCluskey J
Department of Medicine, University of Melbourne, St Vincent's Hospital, Vic., Australia.
Aust N Z J Med. 1991 Jun;21(3):307-13. doi: 10.1111/j.1445-5994.1991.tb04695.x.
The identification of clonal rearrangements of the immunoglobulin heavy chain and T cell receptor beta chain gene loci by Southern blot analysis has led to advances in the diagnosis and classification of lymphoproliferative disorders. This paper reviews our experience with this technique over a three and a half year period. Specimens from 99 patients with suspected haematological malignancy were tested for leucocyte immunophenotype and immunoglobulin or T cell receptor gene rearrangement. Genotyping provided evidence of clonality in malignancies from 28 patients and demonstrated malignant cell lineage in eight patients not formally deduced from immunophenotyping alone. Our findings suggest that this technique can be employed in conjunction with immunophenotyping to aid in the determination of malignant cell lineage derivation and identification of malignant cell clonality, as well as potentially estimating the extent of disease, detecting relapse, and monitoring disease progression.
通过Southern印迹分析鉴定免疫球蛋白重链和T细胞受体β链基因位点的克隆重排，推动了淋巴增殖性疾病诊断和分类的进展。本文回顾了我们在三年半时间里使用该技术的经验。对99例疑似血液系统恶性肿瘤患者的标本进行白细胞免疫表型和免疫球蛋白或T细胞受体基因重排检测。基因分型为28例恶性肿瘤患者的克隆性提供了证据，并在8例仅通过免疫表型分析未正式推断出恶性细胞系的患者中证实了恶性细胞系。我们的研究结果表明，该技术可与免疫表型分析结合使用，以帮助确定恶性细胞系的来源和恶性细胞克隆性，以及潜在地评估疾病范围、检测复发和监测疾病进展。
