Labanauskiene Jūrate, Satkauskas Saulius, Kirveliene Vida, Venslauskas Mindaugas, Atkocius Vydmantas, Didziapetriene Janina
Scientific Research Center, Institute of Oncology, Vilnius University, Vilnius, Lithuania.
Medicina (Kaunas). 2009;45(5):372-7.
The aim of our study was to determine if electroporation could improve the efficacy of photodynamic tumor therapy. A disadvantage of photodynamic therapy is a slow and in some cases insufficient accumulation of photosensitizer in tumor tissue, which could restrict the achievement of an efficient dose. Under the action of electric pulses, cells undergo membrane electroporation, which results in an increased permeability to various exogenous molecules. In this study, murine hepatoma MH22A cells were exposed to light in vitro in the presence of a photosensitizer, either chlorin e6 or aluminum phthalocyanine tetrasulfonate, following electroporation. Accumulation of the photosensitizers was registered by fluorescence microscopy. Cell viability was determined by the MTT assay. Our results demonstrate that electroporation improves an access of chlorin e6 and aluminum phthalocyanine tetrasulfonate to MH22A cells. Electroporation in combination with photosensitization significantly reduces viability of the treated cells even at low doses of photosensitizers.
我们研究的目的是确定电穿孔是否能提高光动力肿瘤治疗的疗效。光动力疗法的一个缺点是光敏剂在肿瘤组织中的积累缓慢，在某些情况下甚至不足，这可能会限制有效剂量的达成。在电脉冲的作用下，细胞会发生膜电穿孔，这会导致对各种外源分子的通透性增加。在本研究中，小鼠肝癌MH22A细胞在电穿孔后，于体外在存在光敏剂（二氢卟吩e6或四磺基铝酞菁）的情况下接受光照。通过荧光显微镜记录光敏剂的积累情况。通过MTT法测定细胞活力。我们的结果表明，电穿孔改善了二氢卟吩e6和四磺基铝酞菁进入MH22A细胞的情况。即使在低剂量光敏剂的情况下，电穿孔与光致敏相结合也能显著降低处理后细胞的活力。
