Atashi Amir, Nadri Samad, Hafizi Maryam, Soleimani Masoud
Hematology Department, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
J Artif Organs. 2009;12(2):118-22. doi: 10.1007/s10047-009-0450-y. Epub 2009 Jun 18.
Conventional methods for differentiation of chondroprogenitor cells on plastic plates face several problems that hinder the application of this method for the treatment of chondrogenic injury. This work focused on the effect of poly-L-lysine (PLL)-coated plastic surfaces and fetal calf serum concentration on the chondroprogenitor cells. In the present study, cartilage was isolated from the articular cartilages of sheep and the cells were seeded on PLL-coated plates in various serum concentrations. Histochemical analysis was used to determine chondrogenic differentiation of the cells. According to our results, the cells formed three-dimensional masses and chondrogenic cells. In the present investigation, the best culture conditions for maximum proliferation of isolated cells were examined. Taken together, the results indicated that PLL may have some effect on the adhesive properties of chondroprogenitor cells and could be used for cartilage engineering.
在塑料平板上分化软骨祖细胞的传统方法面临若干问题，这些问题阻碍了该方法在软骨损伤治疗中的应用。这项工作聚焦于聚-L-赖氨酸（PLL）包被的塑料表面和胎牛血清浓度对软骨祖细胞的影响。在本研究中，从绵羊的关节软骨中分离出软骨，将细胞接种在不同血清浓度的PLL包被平板上。采用组织化学分析来确定细胞的软骨分化情况。根据我们的结果，细胞形成了三维团块和软骨细胞。在本研究中，考察了使分离细胞实现最大增殖的最佳培养条件。综合来看，结果表明PLL可能对软骨祖细胞的黏附特性有一定影响，可用于软骨工程。
