Mukai K, Imai M, Shimada H, Okada Y, Ogishima T, Ishimura Y
Department of Biochemistry, School of Medicine, Keio University, Tokyo, Japan.
Biochem Biophys Res Commun. 1991 Nov 14;180(3):1187-93. doi: 10.1016/s0006-291x(05)81321-x.
Two rat genomic clones, one for cytochrome P-450aldo and the other for P-450(11) beta, were isolated and characterized. The two genes, encoding structurally homologous proteins, were closely similar in their intron-exon organizations. Their 5'-flanking regions, however, contained only a few homologous regions. A putative cyclic AMP responsive element, TGACGTGA, was found in the P-450aldo gene, but this sequence was altered at two positions in the P-450(11) beta gene. S1 nuclease protection assay revealed a single transcription initiation site for the P-450aldo gene, while multiple sites were found for the P-450(11) beta gene. These results suggest that transcriptional regulation of the rat P-450aldo and P-450(11) beta genes is due to differences in the sequences of their 5'-flanking regions.
分离并鉴定了两个大鼠基因组克隆，一个是关于细胞色素P - 450aldo的，另一个是关于P - 450(11)β的。这两个编码结构同源蛋白的基因，其内含子 - 外显子组织非常相似。然而，它们的5'侧翼区域仅包含少数同源区域。在P - 450aldo基因中发现了一个假定的环磷酸腺苷反应元件TGACGTGA，但该序列在P - 450(11)β基因的两个位置发生了改变。S1核酸酶保护试验揭示了P - 450aldo基因有一个单一的转录起始位点，而P - 450(11)β基因有多个转录起始位点。这些结果表明，大鼠P - 450aldo和P - 450(11)β基因的转录调控是由于它们5'侧翼区域序列的差异。
