Guo Qingfa, Shi Shuai, Wang Xiuhong, Kan Bing, Gu Yingchun, Shi Xingyu, Luo Feng, Zhao Xia, Wei Yuquan, Qian Zhiyong
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, China.
Int J Pharm. 2009 Sep 8;379(1):82-9. doi: 10.1016/j.ijpharm.2009.06.010. Epub 2009 Jun 17.
In this paper, a novel biodegradable poly(ester amine) (PEA) copolymer was successfully prepared from low-molecular-weight polyethyleneimine (PEI, Mn=1800) and poly(epsilon-caprolactone)-Pluronic-poly(epsilon-caprolactone) (PCFC) copolymers. According to the results of agarose gel electrophoresis, particle sizes and zeta potential measurement and transfection efficiency, these PEA copolymers showed great ability to condense plasmid DNA effectively into nano-complexes with small particle size (< or =200 nm) and moderate zeta potential (> or =12 mV) at proper polymeric carrier/DNA weight ratio. Compared with low-molecular-PEI (Mn=1800), the obtained PEAs exhibited higher transfection efficiency as well as lower cytotoxicity. These results indicated that such PEAs might have great potential application in gene delivery system.
在本文中，通过低分子量聚乙烯亚胺（PEI，Mn = 1800）与聚（ε-己内酯）-普朗尼克-聚（ε-己内酯）（PCFC）共聚物成功制备了一种新型的可生物降解聚（酯胺）（PEA）共聚物。根据琼脂糖凝胶电泳、粒径和zeta电位测量以及转染效率的结果，这些PEA共聚物在适当的聚合物载体/DNA重量比下，具有很强的能力将质粒DNA有效地凝聚成粒径小（≤200 nm）且zeta电位适中（≥12 mV）的纳米复合物。与低分子量PEI（Mn = 1800）相比，所得的PEA表现出更高的转染效率以及更低的细胞毒性。这些结果表明，此类PEA在基因递送系统中可能具有巨大的潜在应用价值。
