Rejiya C S, Cibin T R, Abraham Annie
Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram 695 581, Kerala, India.
Toxicol In Vitro. 2009 Sep;23(6):1034-8. doi: 10.1016/j.tiv.2009.06.010. Epub 2009 Jun 18.
Cassia tora Linn (Leguminacea) is a medicinal plant traditionally used as laxative, for the treatment of leprosy and various skin disorders. Preliminary phytochemical analysis of leaf showed the presence of polyphenols (3.7 mg gallic acid equivalent per gram dried leaves). The presence of phenolic compound prompted us to evaluate its antioxidant and antiproliferative potential. In the present study C. tora methanolic leaf extract (CTME) was evaluated for its nitric oxide scavenging activity and reducing power assays using Rutin and BHT as standards. The extract was studied for its lipid peroxidation inhibition assay using rat liver and brain. In all assays, a correlation existed between concentration of extract and percentage inhibition of free radical, reducing power and inhibition of lipid peroxidation. The antiproliferative activity of CTME with Cisplatin, anticancer drug was studied using human cervical cancer cells (HeLa). Proliferation of HeLa was measured by MTT assay, cell DNA content by modified diphenylamine method and apoptosis by Caspase 3 activity. The plant extract induced a marked concentration dependent inhibition on proliferation, reduced DNA content and apoptosis in HeLa. These results clearly indicate that C. tora is effective against free radical mediated diseases.
决明（豆科）是一种药用植物，传统上用作泻药，用于治疗麻风病和各种皮肤病。对其叶子进行的初步植物化学分析表明存在多酚（每克干叶含3.7毫克没食子酸当量）。酚类化合物的存在促使我们评估其抗氧化和抗增殖潜力。在本研究中，以芦丁和丁基羟基甲苯为标准，对决明甲醇叶提取物（CTME）的一氧化氮清除活性和还原能力进行了评估。使用大鼠肝脏和大脑对该提取物进行了脂质过氧化抑制试验。在所有试验中，提取物浓度与自由基抑制百分比、还原能力和脂质过氧化抑制之间均存在相关性。使用人宫颈癌细胞（HeLa）研究了CTME与抗癌药物顺铂的抗增殖活性。通过MTT法测定HeLa的增殖，通过改良的二苯胺法测定细胞DNA含量，通过半胱天冬酶3活性测定细胞凋亡。该植物提取物对HeLa的增殖、DNA含量降低和凋亡具有明显的浓度依赖性抑制作用。这些结果清楚地表明决明对自由基介导的疾病有效。
