Hajeri Praveensingh B, Singh Sunit Kumar
Section of Infectious Diseases & Immunobiology, Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad-500007, AP, India.
Drug Discov Today. 2009 Sep;14(17-18):851-8. doi: 10.1016/j.drudis.2009.06.001. Epub 2009 Jun 21.
RNA interference (RNAi) is a novel and essential biological process, as well as a powerful experimental tool with the potential to be used in therapeutic development. RNAi-based strategies have the capability of being able to be driven from bench to bedside. It is very important to develop the precise tools for designing the siRNAs to get the most efficient knockdown of the target genes and to reduce any off-target effects. In this review we have discussed the strategies and parameters required for effective siRNA designing and synthesis, based on already published literature.
RNA干扰（RNAi）是一种新颖且重要的生物学过程，也是一种有潜力用于治疗开发的强大实验工具。基于RNAi的策略有能力从实验室走向临床应用。开发精确的工具来设计小干扰RNA（siRNA）以最有效地敲低靶基因并减少任何脱靶效应非常重要。在本综述中，我们根据已发表的文献讨论了有效设计和合成siRNA所需的策略和参数。
