Biomedical Primate Research Centre, Lange Kleiweg 139, 2288 GJ Rijswijk, The Netherlands.
J Proteomics. 2010 Jan 3;73(3):403-20. doi: 10.1016/j.jprot.2009.06.005. Epub 2009 Jun 21.
Membrane proteomics is concerned with accurately and sensitively identifying molecules involved in cell compartmentalisation, including those controlling the interface between the cell and the outside world. The high lipid content of the environment in which these proteins are found often causes a particular set of problems that must be overcome when isolating the required material before effective HPLC-MS approaches can be performed. The membrane is an unusually dynamic cellular structure since it interacts with an ever changing environment. A full understanding of this critical cell component will ultimately require, in addition to proteomics, lipidomics, glycomics, interactomics and study of post-translational modifications. Devoid of nucleus and organelles in mammalian species other than camelids, and constantly in motion in the blood stream, red blood cells (RBCs) are the sole mammalian oxygen transporter. The fact that mature mammalian RBCs have no internal membrane-bound organelles, somewhat simplifies proteomics analysis of the plasma membrane and the fact that it has no nucleus disqualifies microarray based methods. Proteomics has the potential to provide a better understanding of this critical interface, and thereby assist in identifying new approaches to diseases.
膜蛋白质组学关注的是准确和敏感地识别参与细胞区室化的分子，包括那些控制细胞与外部世界之间界面的分子。这些蛋白质所处的环境脂质含量很高，在分离所需物质之前，必须克服特定的问题，然后才能进行有效的 HPLC-MS 方法。由于膜与不断变化的环境相互作用，因此它是一种非常动态的细胞结构。除了蛋白质组学、脂质组学、糖组学、相互作用组学和翻译后修饰研究外，全面了解这一关键细胞成分最终还需要这些研究。除了骆驼科动物外，哺乳动物物种没有细胞核和细胞器，而且在血液中不断运动，红细胞（RBC）是唯一的哺乳动物氧气转运体。成熟的哺乳动物 RBC 没有内部膜结合的细胞器，这使得质膜的蛋白质组学分析稍微简化了一些，而没有细胞核这一事实使基于微阵列的方法变得不合格。蛋白质组学有可能提供对这一关键界面的更好理解，从而有助于确定治疗疾病的新方法。
