运动期间测量汗液钠和钾流失时区域贴片收集与全身冲洗的比较。
Comparison of regional patch collection vs. whole body washdown for measuring sweat sodium and potassium loss during exercise.
作者信息
Baker Lindsay B, Stofan John R, Hamilton Adam A, Horswill Craig A
机构信息
Gatorade Sports Science Institute, Gatorade Research and Developement, Barrington, Illinois 60010, USA.
出版信息
J Appl Physiol (1985). 2009 Sep;107(3):887-95. doi: 10.1152/japplphysiol.00197.2009. Epub 2009 Jun 18.
This study compared simultaneous whole body washdown (WBW) and regional skin surface (REG) sweat collections to generate regression equations to predict WBW sweat Na(+) concentration ([Na(+)]) and K(+) concentration ([K(+)]) from single- and five-site REG sweat patch collections. Athletes (10 men, 10 women) cycled in a plastic chamber for 90 min in the heat. Before exercise, the subject and bike were washed with deionized water. After the onset of sweating, sterile patches were attached to the forearm, back, chest, forehead, and thigh and removed on saturation. After exercise, the subject and bike were washed with ammonium sulfate solution to collect all sweat electrolyte loss and determine the volume of unevaporated sweat. All individual patch sites and five-site REG (weighted for local sweat rate and body surface area) were significantly (P = 0.000) correlated with WBW sweat [Na(+)]. The equation for predicting WBW sweat [Na(+)] from five-site REG was y = 0.68x + 0.44 [r = 0.97, intraclass correlation coefficient (ICC) = 0.70] and did not differ between sexes. There were sex differences in the regression results between five-site REG and WBW sweat [K(+)] (men: y = 0.74x + 0.30, r = 0.89, ICC = 0.73; women: y = 0.04x + 3.18, r = 0.03, ICC = 0.00). Five-site REG sweat [Na(+)] and [K(+)] significantly overestimated that of WBW sweat (59 +/- 27 vs. 41 +/- 19 meq/l, P = 0.000 and 4.4 +/- 0.7 vs. 3.6 +/- 0.7 meq/l, P = 0.000, respectively). For both sexes, the best sites for predicting WBW sweat [Na(+)] and [K(+)] were the thigh (1 +/- 8 meq/l < WBW, P = 1.000, y = 0.75x + 11.37, r = 0.96, ICC = 0.93) and chest (0.2 +/- 0.3 meq/l > WBW, P = 1.000, y = 0.76x + 0.55, r = 0.89, ICC = 0.87), respectively. In conclusion, regression equations can be used to accurately and reliably predict WBW sweat [Na(+)] and [K(+)] from REG sweat collections when study conditions and techniques are similar to that of the present protocol.
本研究比较了同步全身冲洗（WBW）和局部皮肤表面（REG）汗液采集，以生成回归方程，用于从单部位和五部位REG汗液贴片采集中预测WBW汗液钠（Na⁺）浓度（[Na⁺]）和钾（K⁺）浓度（[K⁺]）。运动员（10名男性，10名女性）在热环境中的塑料舱内骑行90分钟。运动前，用去离子水冲洗受试者和自行车。出汗开始后，将无菌贴片贴在前臂、背部、胸部、前额和大腿上，饱和后取下。运动后，用硫酸铵溶液冲洗受试者和自行车，以收集所有汗液电解质损失并确定未蒸发汗液的体积。所有单个贴片部位和五部位REG（根据局部出汗率和体表面积加权）与WBW汗液[Na⁺]均显著（P = 0.000）相关。从五部位REG预测WBW汗液[Na⁺]的方程为y = 0.68x + 0.44 [r = 0.97，组内相关系数（ICC）= 0.70]，且男女之间无差异。五部位REG和WBW汗液[K⁺]的回归结果存在性别差异（男性：y = 0.74x + 0.30，r = 0.89，ICC = 0.73；女性：y = 0.04x + 3.18，r = 0.03，ICC = 0.00）。五部位REG汗液[Na⁺]和[K⁺]显著高估了WBW汗液的[Na⁺]和[K⁺]（分别为59±27 vs. 41±19 meq/l，P = 0.000和4.4±0.7 vs. 3.6±0.7 meq/l，P = 0.000）。对于男女两性，预测WBW汗液[Na⁺]和[K⁺]的最佳部位分别是大腿（比WBW高1±8 meq/l，P = 1.000，y = 0.75x + 11.37，r = 0.96，ICC = 0.93）和胸部（比WBW高0.2±0.3 meq/l，P = 1.000，y = 0.76x + 0.55，r = 0.89，ICC = 0.87）。总之，当研究条件和技术与本方案相似时，回归方程可用于从REG汗液采集中准确可靠地预测WBW汗液[Na⁺]和[K⁺]。
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